Hoofprint Biome
Hoofprint Biome is a Raleigh-based agricultural biotech startup that develops enzyme-based probiotics and feed additives for cattle to reduce methane emissions and improve feed efficiency, selling directly to farmers and feedlot operators.
- Company typePrivate
- Founded2023
- HeadquartersRaleigh, United States
- Headcount11–50
- GTM typeB2B
- OfferingHardware or Manufacturing
What Hoofprint Biome does
Hoofprint Biome is an agricultural biotechnology startup founded in 2023 in Raleigh, North Carolina, by NC State Ph.D. graduates Kathryn Polkoff (CEO) and Scott Collins (CSO). The company develops enzyme-based feed additives and probiotics designed to modify the cattle rumen microbiome, with the dual objective of reducing methane emissions and improving feed conversion efficiency. Its core technology centers on a probiotic yeast that enters the rumen and releases natural enzymes targeting methane-producing microbes, addressing a problem the company frames as cattle methane accounting for 6% of global greenhouse gas emissions and costing cattle up to 12% of feed energy.
The product is delivered as a feed additive that farmers and feedlot operators incorporate into existing cattle feeding operations. The company's go-to-market motion is enterprise field sales, with direct engagement on cattle farms and feedlot operations for on-farm trials and commercial deployment. Primary customers are cattle farmers and feedlot operators seeking to improve feed efficiency and reduce environmental impact, with a secondary addressable segment being the broader sustainable agriculture sector.
Hoofprint Biome has raised approximately $22.17 million in cumulative funding across a May 2023 seed, two 2023 pre-seed rounds, and an April 2025 Series A led by SOSV with participation from Amazon's Climate Pledge Fund, Breakthrough Energy Fellows, Alexandria Venture Investments, AgriZeroNZ, Good Growth Capital, Ponderosa Ventures, and Twynam. The company operates with 1-10 employees, has no public revenue disclosure, and is currently transitioning from R&D into commercial deployment of its first product line.
Hoofprint Biome firmographics
Firmographics- Name
- Hoofprint Biome
- Legal name
- Hoofprint Biome
- Website
- https://hoofprintbiome.com
- Company type
- Private
- Founded year
- 2023
- Operating status
- Operating
- Headcount range
- 11–50 employees
- Short description
- Hoofprint Biome is a Raleigh-based agricultural biotech startup that develops enzyme-based probiotics and feed additives for cattle to reduce methane emissions and improve feed efficiency, selling directly to farmers and feedlot operators.
- Ownership category
- akta.pro rank
Hoofprint Biome industry classification
Industry- Product category
- Animal Feed Additives
- NAICS
- Animal Food Manufacturing (3111)
- SIC
- Agricultural Prod-Livestock & Animal Specialties (200), Biological Products, (No Disgnostic Substances) (2836)
- akta.pro primary industry
- Medicated & Functional Ruminant Feed (Ionophores, Buffers, Yeast/Probiotics, Mycotoxin Binders) (AFADAAAL)
- akta.pro secondary industries
- Enzymes (Phytase, Xylanase, Protease) (AFADALAD), Immune & Stress Modulators (Beta-glucans, Nucleotides) (AFADAKAH)
Keywords
Where Hoofprint Biome is headquartered
LocationHeadquarters
- HQ city
- Raleigh
- HQ country
- United States
- HQ region
- North America
Offices2 records
Markets served
Hoofprint Biome business model
Business model- GTM type
- B2B
- Offering type
- Hardware or Manufacturing
- Cost components
- Technology or R&D, Supply Chain, Personnel, Operations, Marketing or Sales
Revenue model
- Enzyme-based Feed Additives: The company generates revenue through the sale of enzyme-based feed additives and probiotics for cattle. The product is delivered as a feed additive that farmers incorporate into cattle feed to reduce methane emissions and improve feed efficiency. Currently in commercial deployment and on-farm trial phase.
Go-to-market motion1 record
Distribution channels1 record
Marketing channels2 records
Hoofprint Biome product offering
Product offeringCore offering
Hoofprint Biome develops and sells enzyme-based feed additives (delivered via probiotic yeasts) for cattle that modify the rumen microbiome to reduce methane emissions while improving feed efficiency and nutrient absorption. The product is sold as a feed additive that farmers incorporate into cattle feed, with commercial deployment via direct on-farm trials with cattle farmers and feedlot operators.
Product overview
Hoofprint Biome is a biotech startup developing a single unified product: enzyme-based feed additives delivered via probiotic yeasts that enter cattle's rumen and release natural enzymes. The probiotics and enzymes work together to modify the cattle microbiome, eliminating methane production while improving digestive efficiency and feed conversion. The product represents an integrated biological solution targeting both environmental sustainability (methane reduction) and agricultural productivity (improved yield).
Differentiator
Problem solved
Functional benefit
Products and services
- Hoofprint Biome Enzyme-Based Feed Additives Enzyme-based feed additives and probiotics for cattle that modify the rumen microbiome to significantly reduce methane emissions while improving feed efficiency and nutrient absorption. The probiotic yeast enters the rumen and releases enzymes that target microbes responsible for methane production. Designed for cattle farmers and feedlot operators integrating the additive into existing cattle feed operations.
Quantifiable outcome
- Reduces methane emissions from cattle significantly
- +2 more outcomes
Companies that use Hoofprint Biome
Customer profileSegments2 records
Ideal customer profiles2 records
Hoofprint Biome technology and API
TechnologyTechnology focussed No
API detail
- Has API
- No
- API docs
- API detail
Core technology
AI maturity
App detail
Feature3 records
Hoofprint Biome partnerships and signals
Strategic signalScale indicators6 records
Recent moves5 records
Expansion highlights5 records
Hoofprint Biome competitors and assessment
Company assessmentDirect peers
- Elanco Animal Health: Major animal health company that has commercialized Bovaer (3-NOP), a feed additive that reduces enteric methane emissions in cattle. Direct competitor in the same product category targeting the same cattle farmer customer base.
- CH4 Global: Climate-tech company developing Asparagopsis (red seaweed) feed supplements to reduce cattle methane emissions. Competes in the same methane-reduction feed additive category with a different biological mechanism.
- Mootral: Develops garlic and citrus-based feed supplements (Mootral Ruminant) that reduce enteric methane in cattle. Direct competitor with a natural-extract-based approach to the same problem.
- ArkeaBio: Agtech startup developing a vaccine-based approach to reducing cattle methane emissions through microbiome modulation. Competes in the same methane-reduction space with an immunological rather than feed-additive approach.
- Symborg: Agricultural biotechnology company with a portfolio of microbial solutions, including biostimulants and methane-reduction work in livestock. Comparable biotech-led approach to sustainable crop and livestock inputs.
- Agolin: Develops essential oil blend feed additives (Agolin Ruminant) that improve feed efficiency and reduce methane in cattle. Direct competitor with a botanical-based mechanism targeting the same on-farm use case.
- Lallemand Animal Nutrition: Global leader in yeast and bacteria-based feed additives for livestock, including rumen-specific probiotics. Comparable in delivery mechanism (yeast-based products for cattle) and customer base.
Emerging players
- Number 8 Bio: Synthetic biology startup developing feed additives to reduce livestock methane emissions. Closely comparable early-stage peer using engineered biology for the same end customer.
Broad incumbents
- dsm-firmenich: Global nutrition and health major with deep animal nutrition portfolio and Bovaer partnership. Large incumbent serving the same cattle feed additive market with much broader product and geographic scope.
- Cargill: Major agribusiness with a large animal nutrition business and active sustainability/regenerative agriculture programs. Serves the same cattle feedlot customer base with broader feed and sustainability offerings.
Market position
Strengths4 records
Weaknesses4 records
Competitive moat3 records
Key risks5 records
Key highlights5 records
Customer concentration
Hoofprint Biome financial estimates
Financial estimateRevenue estimate
Valuation estimate
Hoofprint Biome leadership team
Management profileNumber of profiles
Profiles2 records
Hoofprint Biome funding detail
Funding detailFunding overview
Funding rounds2 records
Investors8 records
Funding detail is available on the Subscription and Enterprise plan.Contact sales →
Hoofprint Biome M&A and investment
M&A and investmentM&A
Investments
M&A and investment is available on the Subscription and Enterprise plan.Contact sales →
Frequently asked questions about Hoofprint Biome
What does Hoofprint Biome do?
Hoofprint Biome develops and sells enzyme-based feed additives (delivered via probiotic yeasts) for cattle that modify the rumen microbiome to reduce methane emissions while improving feed efficiency and nutrient absorption. The product is sold as a feed additive that farmers incorporate into cattle feed, with commercial deployment via direct on-farm trials with cattle farmers and feedlot operators.
Is Hoofprint Biome a public or private company?
Hoofprint Biome is a private company. It is classified as venture growth investor backed and is currently operating.
When was Hoofprint Biome founded?
Hoofprint Biome was founded in 2023. It employs 11 to 50 people.
Where is Hoofprint Biome based?
Hoofprint Biome is headquartered in Raleigh, United States, in the North America region.
How does Hoofprint Biome make money?
One revenue line is on record: enzyme-based Feed Additives.
Who are Hoofprint Biome's main competitors?
Direct peers on record are Elanco Animal Health, CH4 Global, Mootral, ArkeaBio, Symborg, Agolin and Lallemand Animal Nutrition. Number 8 Bio is listed as an emerging player. Broad incumbents are dsm-firmenich and Cargill.
Does Hoofprint Biome have an API?
No public API is recorded for Hoofprint Biome.
What industry is Hoofprint Biome in?
Hoofprint Biome's product category is Animal Feed Additives. Its primary akta.pro industry code is AFADAAAL, Medicated & Functional Ruminant Feed (Ionophores, Buffers, Yeast/Probiotics, Mycotoxin Binders), with a secondary code of AFADALAD, Enzymes (Phytase, Xylanase, Protease). Its NAICS code is 3111 and its SIC code is 200.