What Immunostep does

Immunostep is a Spanish biotechnology company founded in 2001 and headquartered at the Cancer Research Center (CIC) on the University of Salamanca campus in Salamanca, Spain. Operating as IMMUNOSTEP, S.L. (tax ID B-37373784), the company develops and manufactures flow cytometry reagents and diagnostics, including oncohematology antibodies (IREM2, TCR CB1, TdT, KAPPA, LAMBDA), multicolor cocktails, diagnostic kits (HeMoStep, Stem Cell Enumeration, Mesenchymal Stem Cell, ErythroStep EMA, BCR/ABL), Euroflow-standardized reagents, and a separate exosome product line built around the ExoStep detection platform, SEC isolation columns, capture beads, and lyophilized reference standards. The company's proprietary Fluorescent Streptavidin Dextran technology uses dextran-fluorochrome-streptavidin complexes to deliver improved antigen recognition, signal amplification, and detection of minority populations below 0.5%, distinguishing it from conventional fluorescent conjugates.

The company's product portfolio also includes CE-IVD marked diagnostic kits for the EU clinical market alongside RUO reagents for research use, plus specialty kits (BasoStep, ThromboStep), accessory reagents (Annexin V apoptosis kits, FcR blocking reagent, StepCount), and distributed Bio-Rad StarBright dye-conjugated antibodies. Revenue is generated through two streams: direct e-commerce sales of catalog products with worldwide shipping (single reagents €142-€656, diagnostic kits €225-€1,990, specialty kits up to €1,250) and quote-based custom services spanning peptide synthesis, protein production, monoclonal/polyclonal antibody development, OEM/bulk production, conjugation, and custom exosome work. Customers are primarily clinical diagnostics laboratories (hematological malignancies, transplant compatibility), life science research institutions (biomarker discovery, exosome research), and, secondarily, cell and gene therapy companies (CAR-T development and monitoring). All purchases require registration as a healthcare professional or authorized research institution.

The company has raised approximately $1.34M from Alentia in May 2016 as its only disclosed institutional funding event. Leadership comprises co-founder and CEO Ricardo Jara alongside technical manager Alfredo Conde. The company operates with 11-50 employees and maintains a multilingual (English/Spanish) web storefront as its primary go-to-market channel, supplemented by direct inquiry forms for custom services.

Name

Immunostep

Legal name

IMMUNOSTEP, S.L.

Website

https://immunostep.com

Company type

Private

Founded year

2001

Operating status

Operating

Headcount range

11–50 employees

Short description

Immunostep is a Salamanca, Spain-based biotechnology company founded in 2001 that develops flow cytometry reagents, diagnostic kits, and exosome isolation products, serving clinical diagnostics labs, life science research institutions, and cell therapy developers worldwide via direct e-commerce and custom services.

Ownership category

akta.pro rank

Product category

Flow Cytometry Reagents

NAICS

In-Vitro Diagnostic Substance Manufacturing (325413)

SIC

In Vitro & In Vivo Diagnostic Substances (2835)

akta.pro primary industry

Reagents, Chemicals & Assay Kits (Buffers, Antibodies, ELISA, Enzymes) (HLAGAIAI)

akta.pro secondary industries

Specialty & Biomarker Laboratory Services (Immunoassays/Flow/Cytometry) (HLAGAEAB), Biomarker Assay & Reagent Development (LDT/IVD assay design, antibodies/standards/controls) (HLAAAMAL)

Keywords

• Flow cytometry reagents
• Antibody development services
• Exosome isolation products
• Clinical diagnostics kits
• Peptide synthesis services

Headquarters

HQ city

Salamanca

HQ country

Spain

HQ region

Europe

Offices1 record

Markets served

GTM type

B2B

Offering type

Hardware or Manufacturing

Cost components

Personnel, Supply Chain, Technology or R&D, Operations, Marketing or Sales, Infrastructure

Revenue model

1. Product Sales: Direct sales of flow cytometry reagents, antibodies, kits, and exosome products through e-commerce website to healthcare professionals and research institutions worldwide. Products include single reagents, multicolor cocktails, and complete diagnostic kits.
2. Custom Services: Custom biotechnological services including peptide synthesis, protein production, monoclonal/polyclonal antibody development, specialized antibody services (OEM/bulk production, purification, conjugation), and custom exosome services.

Pricing tiers

Go-to-market motion1 record

Distribution channels1 record

Marketing channels4 records

Core offering

Immunostep develops and manufactures flow cytometry reagents, antibodies, multicolor cocktails, diagnostic kits, and exosome isolation and characterization products for clinical diagnostics and life science research. The company also offers custom biotechnological services including peptide synthesis, recombinant protein production, monoclonal and polyclonal antibody development, and custom exosome services.

Product overview

Immunostep is a biotechnology company founded in 2001, based in Salamanca, Spain, specializing in flow cytometry reagents and custom services. The company offers a comprehensive portfolio of products organized into four main categories: (1) Oncohematology products including single reagent antibodies (IREM2, TCR CB1, TdT, KAPPA, LAMBDA), multicolor cocktails (P.NH, TBNK), diagnostic kits (HeMoStep, Stem Cell Enumeration, Mesenchymal Stem Cell, ErythroStep EMA, BCR/ABL Protein), CAR T-cell support products, and Euroflow-standardized reagents; (2) Immunology products including FcεR1, Ig subclasses, crossmatch determination (FCXM), SARS-CoV-2 ELISA kits, BasoStep, and ThromboStep; (3) Exosome products featuring the ExoStep detection platform, isolation columns (SEC), capture beads, precipitation solutions, lyophilized standards (CD63, CD9, CD81), and Fluorescent Streptavidin Dextrans for enhanced detection; (4) Accessory reagents including apoptosis kits (Annexin V), cell viability/cell cycle reagents (7AAD, PI), INTRACELL permeabilization kit, FcR blocking reagent, and StepCount absolute count system. The company also provides extensive custom services including peptide production, protein services, monoclonal/polyclonal antibody development, specialized antibody services (OEM, purification, conjugation), and custom exosome services. All products are designed for flow cytometry applications in clinical research and diagnostics.

Differentiator

Problem solved

Functional benefit

Products and services

• Oncohematology Single Reagent Antibodies Individual antibodies including IREM2, TCR CB1 (JOVI.1), TCR CB2 (SAM.2.rMAb), TdT, KAPPA, and LAMBDA for oncohematology flow cytometry diagnostics.
• Multicolor Antibody Cocktails Pre-mixed multicolor antibody cocktails for flow cytometry including P.NH, TBNK, and CD3-FITC/CD8-PE/CD45-PerCP/CD4-APC combinations for immunophenotyping.
• HeMoStep Kit Kit for cerebrospinal fluid (CSF) contamination detection in hematology applications.
• Stem Cell Enumeration Kit Kit for stem cell enumeration in oncohematology research and clinical workflows.
• Mesenchymal Stem Cell Kit Kit for identification and analysis of mesenchymal stem cells by flow cytometry.
• CAR T-cell Products Antibodies and reagents supporting CAR T-cell research, manufacturing characterization, and monitoring workflows.
• Euroflow-standardized Reagents Standardized flow cytometry reagents following Euroflow consortium protocols, including IREM2-APC, CD45 (Clone HI30), Lambda-APC-C750, CD64-PE, CD185-APC, and CD36 PerCP-Cyanine5.5 for immunophenotyping.
• FcεR1 Antibody Fc epsilon receptor 1 antibody for allergy and immunology research applications.
• Ig Subclass Antibodies Immunoglobulin subclass antibodies including IgA1 and IgA2 for immunology research.
• Cross Match Determination (FCXM / XMstep) Flow cytometry-based crossmatch assay for transplant compatibility testing.
• BasoStep Kit Kit for basophil activation and analysis in allergy and immunology research.
• ThromboStep Kit Kit for thrombocyte (platelet) analysis in hematology research.
• BCR/ABL Protein Kit Kit for BCR/ABL protein detection in chronic myeloid leukemia research.
• ErythroStep EMA Kit Kit for hereditary spherocytosis diagnosis using eosin-5-maleimide (EMA) binding assay.
• SARS-CoV-2 ELISA Kits COVID-19 diagnostic and research kits including SARS-CoV-2 MPro ELISA (IgA, IgG), Multiantigen IgG+IgA+IgM assay, and Spike ELISA IgG.
• ExoStep Platform Flow cytometry-based platform for detection and characterization of exosomes using capture beads and standards.
• Exosome Isolation Columns (SEC) Size-exclusion chromatography columns for isolating exosomes from biological samples.
• Exosome Capture Beads Bead-based technology for capturing and concentrating exosomes from various sample types for downstream analysis.
• Exosome Precipitation Solutions Precipitation-based reagent kits for exosome isolation from biofluids and cell culture media.
• Lyophilized Exosome Standards Stabilized exosome reference materials including fluorescent variants, cell line-derived, and serum-derived standards expressing CD63, CD9, and CD81 markers.
• Fluorescent Streptavidin Dextrans Proprietary fluorescent dextran conjugates (PE, FITC, APC) providing signal amplification and improved antigen recognition for flow cytometry applications including antigen-specific B-cell detection and CAR T-cell monitoring.
• Apoptosis Kits and Tools Kits for apoptosis detection including Annexin V Binding Buffer (10X) and CFBlue Annexin V Apoptosis Detection Kit with 7-AAD.
• Cell Viability and Cell Cycle Analysis Reagents Reagents for cell viability assessment and cell cycle analysis including 7AAD Solution, PI Solution, and PI/RNASE Solution.
• StarBright Fluorescent Dye Antibodies StarBright fluorescent dye conjugated antibodies offering enhanced brightness and stability for flow cytometry detection.
• Peptide Production Services Custom peptide design and synthesis services for research and OEM applications.
• Protein Services Recombinant protein production and processing services including expression, purification, and freeze drying.
• Monoclonal and Polyclonal Antibody Development Services Custom antibody development services including hybridoma freezing, storage, isotyping, purification, conjugation, and lyophilization for monoclonal and polyclonal antibodies.
• Specialized Antibody Services OEM/bulk antibody production, purification, conjugation, mycoplasma testing, and decontamination services.
• Custom Exosome Services Custom exosome isolation, purification, and characterization services.

Segments3 records

Ideal customer profiles3 records

Technology focussed Yes

API detail

Has API

No

API docs

API detail

Core technology

AI maturity

App detail

Feature6 records

Scale indicators1 record

Recent moves6 records

Expansion highlights6 records

Direct peers

• BD Biosciences: BD Biosciences is the leading supplier of flow cytometry instruments and reagents, including antibodies, multicolor kits, and clinical immunophenotyping panels. Directly comparable as a flow cytometry reagent provider serving the same clinical diagnostics and research customer base as Immunostep, but at vastly greater scale.
• Beckman Coulter Life Sciences: Beckman Coulter (a Danaher company) offers a broad portfolio of flow cytometry antibodies, reagents, and standardized clinical panels, including Euroflow-aligned products. It is one of Immunostep's most direct competitors in clinical flow cytometry reagents.
• Bio-Rad Laboratories: Bio-Rad manufactures flow cytometry antibodies, kits, and quality controls used in research and clinical labs, including the StarBright dye line also distributed by Immunostep. It is a direct peer across both reagent supply and clinical/research flow applications.
• Miltenyi Biotec: Miltenyi Biotec is a specialist in cell separation, flow cytometry reagents, and CAR-T/cell therapy workflow products. It competes head-on with Immunostep in CAR-T monitoring reagents and clinical flow cytometry consumables.
• Cell Signaling Technology: Cell Signaling Technology produces high-quality validated antibodies and flow cytometry reagents, particularly for immunology and signaling research. It is comparable to Immunostep in antibody quality positioning and research-customer focus.
• STEMCELL Technologies: STEMCELL Technologies supplies reagents and kits for stem cell research, immunology, and cell therapy workflows, including flow cytometry products. It is comparable to Immunostep in serving academic and cell-therapy researchers with specialty reagent kits.
• System Biosciences (SBI): SBI specializes in exosome isolation, characterization, and engineered exosome tools, directly overlapping with Immunostep's ExoStep platform and exosome standards portfolio. It is one of the closest direct peers in the exosome reagent niche.

Broad incumbents

• Thermo Fisher Scientific: Thermo Fisher, through its Invitrogen and eBioscience brands, sells a massive catalog of flow cytometry antibodies, kits, and exosome research tools. It overlaps broadly with Immunostep's catalog but competes across a far wider life sciences portfolio rather than as a flow-cytometry specialist.
• Abcam: Abcam is one of the largest antibody reagent suppliers globally, serving research and diagnostic customers with a catalog comparable in breadth to Immunostep's antibody offerings. It is a broad incumbent rather than a flow-cytometry-only specialist.
• Sysmex: Sysmex is a global leader in hematology diagnostics, including flow cytometry-based clinical immunophenotyping. It overlaps with Immunostep's oncohematology and stem cell enumeration kits but operates at a much broader clinical-diagnostics scale.

Market position

Strengths4 records

Weaknesses4 records

Competitive moat5 records

Key risks6 records

Key highlights7 records

Customer concentration

• Xx.com/Immunostep
• Facebookfacebook.com/immunostepsl
• LinkedInlinkedin.com/company/immunostep

Revenue estimate

Valuation estimate

Number of profiles

Profiles2 records

Funding overview

Funding rounds1 record

Investors1 record

Funding detail is available on the Subscription and Enterprise plan.Contact sales →

M&A

Investments

M&A and investment is available on the Subscription and Enterprise plan.Contact sales →

Frequently asked questions about Immunostep