What Amplifye does

Amplifye is a biotechnology company that commercializes a proprietary acid-activated protease enzyme called P24, designed to enhance protein digestion and absorption. The enzyme is derived from the Kumamolisin family of proteases discovered in acidic Japanese hot springs and was developed through Nobel Prize-winning research at UC Davis. Amplifye produces P24 via a controlled precision fermentation process using an E. coli host with a lactose-based induction system; the enzyme's self-purifying properties eliminate the need for traditional chromatography, simplifying manufacturing. Production is based in India while commercial operations are run from Davis, California.

The company's core product, AmplifyeP24, is sold direct-to-consumer through its e-commerce website at $58 per month on a subscription basis, with one-time purchase options and a 30-day money-back guarantee. Marketing targets fitness-oriented consumers, women in perimenopause, GLP-1 medication users, and metabolic health-conscious individuals, supported by third-party bioaccessibility data showing 1.5x to 4.8x protein availability gains across 20 protein sources. A potential B2B ingredient supply channel is emerging through a strategic investment from Magdalena's ventures arm, which is positioning Amplifye within a broader precision fermentation portfolio that could supply P24 to downstream CPG and supplement manufacturers. The company holds a U.S. patent (issued 2024) covering blood sugar regulation methods using S53 family proteases.

Name

Amplifye

Legal name

Amplifye

Website

https://amplifyeme.com

Company type

Private

Operating status

Operating

Headcount range

11–50 employees

Short description

Amplifye commercializes P24, an acid-activated protease enzyme developed from UC Davis research, sold as a direct-to-consumer dietary supplement ($58/month) that claims to double protein absorption. The company serves fitness, perimenopause, GLP-1, and metabolic health consumers and is exploring B2B enzyme supply via a strategic partnership with Magdalena.

Ownership category

akta.pro rank

Product category

Nutritional Supplements (Protein Digestion Enzymes)

NAICS

Food (Health) Supplement Retailers (456191)

SIC

Medicinal Chemicals & Botanical Products (2833)

akta.pro primary industry

Probiotics & Digestive Health Supplements (CRADAGAF)

akta.pro secondary industry

Therapeutic Enzymes & Enzyme Replacement Therapies (ERT) (HLAAAAAK)

Keywords

• Enzyme supplements
• Protein digestion
• Precision fermentation
• Nutritional supplements
• Digestive enzymes

Headquarters

HQ city

Los Angeles

HQ country

United States

HQ region

North America

Offices1 record

Markets served

GTM type

B2C

Offering type

Hardware or Manufacturing

Cost components

Supply Chain, Operations, Marketing or Sales, Technology or R&D, Personnel

Revenue model

1. P24 Enzyme Subscriptions: Monthly subscription model for P24 enzyme capsules at $58/month. Subscription discount available for first order and recurring charges for one year. Black Friday promotions offered (11/16-12/1/2025).
2. One-Time Product Sales: Single-bottle purchases of P24 enzyme and accessory products like The Travel Case ($45). Customers can purchase individual items without subscription commitment.
3. B2B Enzyme Supply: Potential B2B supply of P24 as an ingredient to downstream CPG and supplement manufacturers. Magdalena's investment indicates strategic interest in enzyme supply partnerships.

Pricing tiers

Go-to-market motion2 records

Distribution channels1 record

Marketing channels5 records

Core offering

Amplifye develops and sells P24, a proprietary acid-activated protease enzyme (S53/Kumamolisin family) that activates in the stomach (pH ~2) to hydrolyze dietary proteins before they reach the small intestine, increasing bioaccessible protein by up to 4.8x depending on the source. The enzyme is produced via precision fermentation with a self-purifying process and sold as a capsule supplement (AmplifyeP24) directly to consumers through amplifyeme.com at $58/month subscription or one-time purchase, with a complementary aluminum Travel Case accessory sold at $45.

Product overview

Amplifye is a consumer health and nutrition company offering a single core product—the AmplifyeP24 (P24) enzyme supplement—paired with a travel accessory. The flagship P24 enzyme is a patented protease that enhances protein digestion and absorption, designed to double the amount of amino acids the body can access from food. The company also sells The Travel Case, an aluminum capsule holder for portability. All products are sold directly through amplifyeme.com with subscriptions available at $58/month.

Differentiator

Problem solved

Functional benefit

Products and services

• AmplifyeP24 (P24 Enzyme) A protease enzyme supplement (capsule form) that enhances protein digestion and absorption by activating in the stomach's acidic environment to break down dietary proteins into peptides and amino acids before they reach the small intestine. Marketed to health-conscious adult consumers including athletes, women in midlife/perimenopause, GLP-1 users, and metabolic health-focused individuals. Sold by subscription at $58/month or as a one-time purchase, with free US shipping and a 30-day money-back guarantee.
• The Travel Case A limited-edition aluminum pill case designed for traveling with P24 capsules. TSA-approved compact dimensions (2.34" L x 1.21" W x 0.91" H) that fit approximately 20 capsules comfortably. Sold as a one-time purchase at $45.

Quantifiable outcome

• Can double protein absorption with one daily capsule
• +3 more outcomes

Named customers5 records

Segments5 records

Ideal customer profiles4 records

Technology focussed Yes

API detail

Has API

No

API docs

API detail

Core technology

AI maturity

App detail

Feature3 records

Partnerships

Two partnerships are on record, tiered core.

• BiorbiscoreStrategic or Co-development PartnerThird-party biotech lab partnered with Amplifye to rigorously evaluate the effect of P24 on protein digestion and amino acid bioaccessibility. Researchers applied validated methods including the TNBS assay and HPLC with fluorescence detection using the INFOGEST Model 2.0 (industry-standard stomach digestion simulation) across 19 protein sources.
• UC DaviscoreStrategic or Co-development PartnerNobel Prize-winning research collaboration with UC Davis researchers who discovered the enzyme in Japanese hot springs and developed it into P24. The research team identified the enzyme's potential to transform how we consume protein and supported the technology transfer to commercial development.

Scale indicators4 records

Recent moves6 records

Expansion highlights6 records

Direct peers

• Athletic Greens (AG1): AG1 is a premium DTC daily nutritional drink subscription (~$70-$80/month) covering foundational health, including digestion, gut health, and protein absorption. Directly comparable as a premium-tier subscription supplement brand competing for the same health-conscious consumer wallet share.
• Seed Health: Seed sells scientifically-formulated probiotic and digestive health supplements via DTC subscription with strong microbiome-science branding. Comparable to Amplifye as a premium, science-led digestive health subscription brand.
• Oobli: Oobli is a precision fermentation company producing sweet proteins and functional ingredients; also a Magdalena Ventures portfolio company alongside Amplifye. Comparable as a fellow precision-fermentation startup sharing investors, technology platform, and manufacturing roadmap.
• VinZymes: VinZymes is a precision fermentation enzyme company and a fellow Magdalena Ventures portfolio company. Highly comparable in business model, technology base, and investor set, though operating in industrial enzyme applications rather than consumer supplements.
• Ritual: Ritual is a premium DTC supplement brand built on traceability, clinical-grade ingredients, and subscription. Comparable to Amplifye as a science-led, design-forward DTC supplement brand competing in the same consumer segment.
• Care/of: Care/of is a personalized DTC vitamin and supplement subscription brand. Comparable as a digital-native subscription supplement company targeting health-conscious consumers with content-led acquisition.

Emerging players

• Pendulum Therapeutics: Pendulum develops targeted probiotic and microbiome products for metabolic health, including glucose management. Comparable to Amplifye on the metabolic-health and digestive-overlap axis, though operating via microbial strains rather than enzymatic protein breakdown.
• Levels Health: Levels sells a continuous glucose monitor and metabolic health insights subscription. Comparable in the metabolic-health consumer segment Amplifye targets with its blood-sugar regulation claims, but Levels competes as a data/measurement company rather than a supplement.
• Transparent Labs: Transparent Labs is a science-focused sports nutrition and protein supplement DTC brand. Comparable in the protein-optimization and fitness segments Amplifye serves, but with a broader sports-nutrition portfolio rather than a single enzyme SKU.
• HumanN: HumanN is a science-led nutrition brand targeting vascular, cognitive, and performance health via dietary supplements. Comparable as a clinically-positioned supplement brand, though with cardiovascular rather than protein-digestion focus.

Market position

Strengths5 records

Weaknesses5 records

Competitive moat5 records

Key risks6 records

Key highlights7 records

Customer concentration

• Instagraminstagram.com/amplifye_me

Revenue estimate

Valuation estimate

Number of profiles

Funding overview

Funding rounds

Investors

Funding detail is available on the Subscription and Enterprise plan.Contact sales →

M&A

Investments

M&A and investment is available on the Subscription and Enterprise plan.Contact sales →

Frequently asked questions about Amplifye