What Dojindo does

Dojindo (株式会社同仁化学研究所 / DOJINDO LABORATORIES) is a privately held Japanese biochemical reagent manufacturer founded circa 1946 and headquartered at Kumamoto Techno Research Park in Kumamoto Prefecture, Japan, with a 2026-published 80th-anniversary top message. The company designs, manufactures, and distributes research-use reagents including cell-based assay kits (cell viability, oxidative stress, ferroptosis, cellular senescence, metabolism, mitophagy, mitochondrial function), fluorescent probes, antibody/protein labeling kits, cell staining dyes, and high-purity analytical solvents (Spectrosol, Luminasol) under both the DOJINDO core brand and a portfolio of registered trademarks (SPiDER-βGal, FerroOrange, HiLyte Fluor, Cell Counting Kit-8, Dead Cell Makeup, Lipi-Green/Red).

The product technology rests on proprietary wet-chemistry platforms: NHS-ester-based antibody/protein labeling that avoids reduction treatment (preserving activity of reduction-sensitive enzymes such as ALP), WI (Washing and Imaging) Solution for probe-leakage suppression in uptake assays, MT-1 MitoMP Detection for fixation-stable mitochondrial membrane potential measurement, and pre-formulated TBARS substrate for MDA quantification. The portfolio serves life science researchers across academia and industry, with horizontal segmentation across use cases rather than vertical specialization, and includes dedicated sub-segments for food science (antioxidant/ACE inhibition kits) and aging research (senescence detection).

Dojindo operates a two-tier go-to-market: in Japan, products flow primarily through Fujifilm Wako Pure Chemical Corporation (primary nationwide distributor) supplemented by a network of over 100 regional sales agents covering all prefectures, with direct sales available via the company website and a free technical support hotline; internationally, the wholly-owned US subsidiary Dojindo Molecular Technologies, Inc. (Maryland) handles sales and English-language technical support. Revenue is generated through per-unit catalog sales priced ¥7,700-¥92,000 per kit (e.g., Cell Counting Kit-8, glucose/amino acid/fatty acid uptake kits, SOD and ACE kits, senescence plate assays), with no evidence of subscription, enterprise licensing, or recurring contract structures. The company is privately held, has no disclosed funding rounds, and no public listing.

Name

Dojindo

Legal name

株式会社同仁化学研究所

Website

https://dojindo.co.jp

Company type

Private

Founded year

1946

Operating status

Operating

Headcount range

1–10 employees

Short description

Dojindo is a privately held Japanese biochemical reagent manufacturer, founded circa 1946 and headquartered in Kumamoto. It designs and distributes cell-based assay kits, fluorescent probes, antibody labeling reagents, and high-purity solvents for academic and industrial life science researchers via Fujifilm Wako Pure Chemical and over 100 regional sales agents.

Ownership category

akta.pro rank

Product category

Life Science Research Reagents and Assay Kits

NAICS

Research and Development in Biotechnology (except Nanobiotechnology) (541714)

SIC

Miscellaneous Chemical Products (2890)

akta.pro primary industry

Reagents, Chemicals & Assay Kits (Buffers, Antibodies, ELISA, Enzymes) (HLAGAIAI)

Keywords

• Biochemical reagents
• Cell assay kits
• Fluorescent probes
• Life science reagents
• Antibody labeling kits

Headquarters

HQ city

Kumamoto

HQ country

Japan

HQ region

Asia

Offices3 records

Markets served

GTM type

B2B

Offering type

Hardware or Manufacturing

Cost components

Personnel, Supply Chain, Technology or R&D, Operations, Marketing or Sales, Infrastructure

Revenue model

1. Research Reagent Sales: Sale of biochemical reagents, assay kits, fluorescent probes, and cell staining reagents to life science researchers. Products are sold through a nationwide distributor network (Fujifilm Wako as primary distributor) and direct sales. Pricing is per unit (kit/reagent) with catalog-listed prices.

Pricing tiers

Go-to-market motion1 record

Distribution channels3 records

Marketing channels6 records

Core offering

Dojindo manufactures and sells a portfolio of research-use biochemical reagents and assay kits for life science research, including cell viability and cytotoxicity kits (Cell Counting Kit-8/CCK-8), cellular metabolism assays (glucose, amino acid, fatty acid uptake kits), oxidative stress reagents (SOD-WST, MDA, ROS, GSH/GSSG), ferroptosis markers (FerroOrange), cellular senescence kits (SPiDER-βGal), mitochondrial and mitophagy detection kits, cell staining dyes, antibody and protein labeling kits (NHS-ester, maleimide, biotin, HRP, ALP, HiLyte Fluor, R-PE, APC, ICG), biofilm assays, and high-purity solvents (Spectrosol, Luminasol) plus water quality testing reagents. The company serves academic, pharmaceutical, and biotech research customers through its US subsidiary and a nationwide Japanese distribution network led by Fujifilm Wako Pure Chemical.

Product overview

Dojindo (同仁化学研究所 / DOJINDO LABORATORIES) is a Japanese biochemical reagent manufacturer headquartered in Kumamoto, Japan. The company operates as both a manufacturer and distributor (総販売元) of research-use reagents, serving life science research institutions and pharmaceutical companies. The product portfolio is organized into two primary categories: (1) Functional biochemical reagents including assay kits for cell viability (Cell Counting Kit-8, CK04), oxidative stress (SOD Assay Kit-WST, S311; MDA Assay Kit, M496; ROS Assay Kit), ferroptosis (GSSG/GSH Quantification Kit II, G263; FerroOrange, F374), cellular senescence (Cellular Senescence Plate Assay Kit-SPIDER-betaGal, SG05; Cellular Senescence Detection Kit-SPIDER-betaGal, SG03), metabolism (Glucose Uptake Assay Kit series in Blue/Green/Red UP01-03; Amino Acid Uptake Assay Kit, UP04; Fatty Acid Uptake Assay Kit, UP07), autophagy/mitophagy (Mitophagy Detection Kit, MD01), and mitochondrial function (MT-1 MitoMP Detection Kit, MT13), along with cell staining dyes (LysoPrime series, Dead Cell Makeup series, DAPI), antibody/protein labeling kits (LK series with fluorescein, biotin, peroxidase, ALP, HiLyte Fluor, R-PE, APC, ICG), and biofilm research reagents (B601, B603, B606); and (2) General analytical reagents including water quality testing kits (residual chlorine, COD, heavy metals), high-purity solvents (Spectrosol, Luminasol), buffers, chelating agents, colorimetric reagents, and ion-selective electrodes. The company distributes its products through Fujifilm Wako Pure Chemical as the primary distributor and a nationwide network of authorized sales agents. Technical services include protocol documentation, custom manufacturing (受託サービス), and a Forum event series.

Differentiator

Problem solved

Functional benefit

Brands

• Dojindo Molecular Technologies: US-based subsidiary for research reagents and assay kits

Products and services

• Cell Counting Kit-8 (CK04) Cell proliferation and cytotoxicity assay kit using WST-8 dye for colorimetric measurement of cell viability. Used by life science researchers in cell biology and drug discovery.
• Glucose Uptake Assay Kit-Blue (UP01) Fluorescent glucose analog probe for measuring cellular glucose uptake via fluorescence microscopy or flow cytometry. For life science researchers studying cellular metabolism.
• Glucose Uptake Assay Kit-Green (UP02) Green fluorescent glucose analog probe enabling plate reader measurement of cellular glucose uptake with reduced probe leakage via WI Solution technology. For metabolism researchers.
• Glucose Uptake Assay Kit-Red (UP03) Red fluorescent glucose analog probe for multi-color imaging and plate reader measurement of cellular glucose uptake. For metabolism and multi-color imaging researchers.
• Amino Acid Uptake Assay Kit (UP04) Assay kit for detecting cellular amino acid uptake via BPA analog transported through LAT transporters, detectable by fluorescence microscopy, plate reader, and flow cytometry. For metabolism and transport researchers.
• Fatty Acid Uptake Assay Kit (UP07) Kit measuring cellular fatty acid uptake using a fatty acid analog probe via fluorescence detection with optional Quenching Buffer for simplified washing. For lipid metabolism researchers.
• Antibody Labeling Kit series (LK01–LK55) Complete series of labeling kits for direct antibody/protein labeling with fluorescein, biotin, peroxidase, alkaline phosphatase, HiLyte Fluor dyes, R-phycoerythrin, allophycocyanin, and ICG, available in NH2- and SH-reactive forms including rapid 30-minute labeling options. For immunoassay and protein conjugation researchers.
• LysoPrime Green (L261) Lysosome staining reagent with high specificity and pH resistance for fluorescence microscopy, FCM, and plate reader applications. For cell biology and autophagy researchers.
• LysoPrime Deep Red (L264) Deep red lysosome staining reagent with high specificity and pH resistance for multi-color imaging. For cell biology and autophagy researchers.
• MT-1 MitoMP Detection Kit (MT13) Mitochondrial membrane potential detection kit that maintains fluorescence after PFA fixation, enabling time-lapse monitoring with high sensitivity comparable to TMRE. For mitochondrial function researchers.
• Mitophagy Detection Kit (MD01) Kit using Mtphagy Dye and Lyso Dye to detect mitophagy via fluorescence microscopy, with real-time imaging capability under starvation conditions. For autophagy researchers.
• Biofilm Formation Assay Kit (B601) Kit for measuring biofilm formation quantity and formation inhibition. For microbiology and biofilm researchers.
• Biofilm Viability Assay Kit (B603) Kit for measuring drug efficacy on biofilms. For microbiology and antimicrobial researchers.
• Dead Cell Makeup Blue (C555) Blue dead cell staining reagent with higher retention than propidium iodide (PI) for FCM applications. For cell viability and flow cytometry researchers.
• Dead Cell Makeup Deep Red (C556) Deep red dead cell staining reagent with higher retention than PI for FCM applications. For multi-color flow cytometry researchers.
• CLAMP F405-Signal Boosting (C554) Immunostaining blue fluorescent substrate for signal amplification. For immunohistochemistry and immunostaining researchers.
• Lipid Peroxidation Probe BDP 581/591 C11 (L267) Fluorescent probe for detecting lipid peroxidation in cells. For oxidative stress and ferroptosis researchers.
• ROS Assay Kit -Photo-oxidation Resistant DCFH-DA (R253) Photo-oxidation resistant total ROS detection kit using DCFH-DA. For oxidative stress researchers.
• GSSG/GSH Quantification Kit II (G263) Glutathione quantification kit for measuring GSSG/GSH ratio as a ferroptosis marker. For oxidative stress and ferroptosis researchers.
• MDA Assay Kit (M496) Malondialdehyde measurement kit using TBARS method for detecting lipid peroxidation and oxidative stress/ferroptosis markers; pre-formulated substrate eliminates weighing and heat dissolution steps.
• FerroOrange (F374) Intracellular iron(II) measurement reagent for fluorescence microscopy (registered trademark). For ferroptosis researchers.
• SOD Assay Kit - WST (S311) Superoxide dismutase activity measurement kit using WST-1 tetrazolium salt for antioxidant capacity evaluation, with 100% SOD inhibition measurement range. For oxidative stress researchers and food science applications.
• Liperfluo (L248) Fluorescent probe for detecting lipid peroxides in cells. For oxidative stress and ferroptosis researchers.
• DPPH Antioxidant Assay Kit (D678) Antioxidant capacity measurement kit using DPPH radical scavenging. For food science, nutraceutical, and antioxidant researchers.
• ACE Kit - WST (A502) Angiotensin-converting enzyme (ACE) inhibition assay kit using enzymatic detection of 3-hydroxybutyric acid for food function evaluation. For food science and hypertension-related nutraceutical research.
• Cellular Senescence Plate Assay Kit - SPiDER-βGal (SG05) 96-well plate format kit for quantifying SA-β-galactosidase activity using SPiDER-βGal fluorescent substrate for cellular senescence detection. For aging and senolytic drug discovery researchers.
• Cellular Senescence Detection Kit - SPiDER-βGal (SG03) SA-β-galactosidase detection kit for imaging senescent cells using SPiDER-βGal, compatible with live and fixed cells. For aging research and cellular senescence imaging.
• Lysosomal Acidic pH Detection Kit (L266) Dual-dye kit combining pH-dependent pHLys Red and pH-independent LysoPrime Green for simultaneous lysosome quantity and pH measurement, with Bafilomycin A1 included as inhibitor control. For autophagy and lysosomal function researchers.
• Cellstain DAPI Solution (D523) Ready-to-use 4',6-diamidino-2-phenylindole dihydrochloride (DAPI) solution for nuclear and dead cell staining (CAS 28718-90-3). For general cell biology and microscopy researchers.
• Cell Count Normalization Kit (C544) Nuclear staining kit using Hoechst 33342 for convenient cell count normalization in 96-well plate assays. For assay normalization in high-throughput workflows.
• Residual Chlorine Measurement Kit (SBT series) Water quality analysis reagents for measuring residual chlorine, COD, heavy metals (chromium, copper, iron, nickel), fluorine, cyanide, and water hardness. For environmental and water testing laboratories.
• Spectrosol high-purity solvents High-purity solvent brand for spectroscopy and analytical applications. For analytical and spectroscopic researchers.
• Luminasol high-purity solvents High-purity solvent brand for luminescence and specialized analytical applications. For luminescence and analytical researchers.
• Biofilm TestPiece Assay Kit (B606) Biofilm formation assay kit designed for testing specimen materials. For antimicrobial material testing and microbiology researchers.

Quantifiable outcome

• Glucose uptake assays reduce measurement time compared to 2-NBDG methods while providing higher sensitivity and reproducibility
• +2 more outcomes

Segments3 records

Ideal customer profiles3 records

Technology focussed No

API detail

Has API

No

API docs

API detail

Core technology

AI maturity

App detail

Feature5 records

Partnerships

One partnership is on record.

• Dojindo Molecular Technologies, Inc.coreStrategic or Co-development PartnerUS-based subsidiary company responsible for international sales and marketing of Dojindo products outside Japan. Located in Maryland, USA. Supports global distribution and provides English-language technical support for international customers.

Scale indicators4 records

Recent moves5 records

Expansion highlights4 records

Direct peers

• Enzo Life Sciences: Specialty life science reagent manufacturer offering assay kits, antibodies, and probes for cell biology, oxidative stress, and apoptosis research. Direct competitor in many of Dojindo's core product categories (cell viability, oxidative stress, senescence).
• Cayman Chemical: US-based specialty biochemical manufacturer focused on research reagents for oxidative stress, ferroptosis, lipid peroxidation, and inflammation research — directly overlapping Dojindo's MDA, SOD, GSH/GSSG, and ferroptosis kit lines. Privately-held and comparable in specialization depth.
• Tocris Bioscience: Bio-Techne-owned supplier of life science research reagents including fluorescent probes, assay kits, and biochemical tools for cell biology and neuroscience. Directly comparable in catalog breadth and customer base (academic and pharma researchers).
• Tokyo Chemical Industry (TCI): Japanese specialty chemical manufacturer offering research reagents, organic chemicals, and life science products with global distribution. Comparable in privately-held Japanese chemical/reagent manufacturer profile, broad catalog of research-grade chemicals, and similar customer segments (academic, pharma, biotech).
• Fujifilm Wako Pure Chemical Corporation: Primary nationwide distributor for Dojindo and itself a major Japanese manufacturer of life science reagents, analytical chemicals, and laboratory products. Directly comparable in product categories (assay kits, biochemical reagents, specialty chemicals) and serves the same Japan academic/industrial research customer base.
• Cell Signaling Technology: Privately-held US manufacturer of antibodies, assay kits, and reagents for cell biology and signal transduction research. Comparable in specialization, research-customer focus, and proprietary reagent offerings with strong brand recognition in the research community.
• Nacalai Tesque: Kyoto-based Japanese manufacturer and distributor of life science research reagents, biochemicals, and laboratory chemicals. Closely comparable to Dojindo in product portfolio (reagents, assay kits, cell biology tools) and Japan-domestic distribution model targeting academic and pharma research customers.

Broad incumbents

• Thermo Fisher Scientific: Global life sciences platform with extensive reagent, antibody, and assay kit offerings (Invitrogen, Molecular Probes, Thermo Scientific brands). Overlaps with Dojindo's product categories at scale but operates as a broad incumbent serving all life science customer segments worldwide.
• Merck KGaA (MilliporeSigma / Sigma-Aldrich): Global leader in life science reagents with the broadest catalog in the industry (Sigma-Aldrich, Supelco, Millipore). Offers competitive versions of nearly every product Dojindo sells (assay kits, fluorescent probes, biochemicals) but as part of a much wider portfolio, not specializing in Dojindo's niche categories.

Emerging players

• Dojindo Molecular Technologies: Wholly-owned US subsidiary of Dojindo handling international sales. While technically part of Dojindo Group, it functions in the US market as a regional/niche specialty reagent distributor comparable in scale and product focus to other small US-based specialty reagent firms.

Market position

Strengths4 records

Weaknesses4 records

Competitive moat4 records

Key risks6 records

Key highlights7 records

Customer concentration

• Xtwitter.com/dojindoInfo
• YouTubeyoutube.com/channel/UCajQLfpLdZMhvN4gxG-UgMw
• Facebookfacebook.com/profile.php?id=100070898981748
• LinkedInlinkedin.com/company/dojindo-laboratories-co-ltd-

Revenue estimate

Valuation estimate

Number of profiles

Profiles1 record

Subsidiaries2 records

Funding overview

Funding rounds

Investors

Funding detail is available on the Subscription and Enterprise plan.Contact sales →

M&A

Investments

M&A and investment is available on the Subscription and Enterprise plan.Contact sales →

Frequently asked questions about Dojindo