Affibinder S.r.o.

What Affibinder S.r.o. does

AffiPro s.r.o. is a Czech private limited company (s.r.o.) that designs, manufactures, and sells specialty consumables and instrumentation for proteomics sample preparation in mass spectrometry workflows. The company's portfolio centers on acidic protease columns (Pepsin, Rhizopuspepsin, Nepenthesin-1/2, Aspergillopepsin, AnPep) with distinct cleavage specificities for on-line protein digestion, mixed-phase columns optimized for antibodies and membrane proteins, a PNGase rc glycanase column, the HDX Workstation automation platform for Hydrogen-Deuterium Exchange Mass Spectrometry (HDX-MS), and 3D-printed Affinity MALDI plates functionalized via ambient ion landing. AffiPro sells primarily through a direct e-commerce storefront at eshop.affipro.cz with publicly listed unit pricing of €1,265-€1,980 per column and €250 express cold-chain shipping, supplemented by direct inquiry, sales team outreach, and a heavy conference presence at ASMS, HDX-MS, and the Clinical & Translational Omics Symposium.

AffiPro is a formal May 2025 spin-off of the Institute of Microbiology of the Czech Academy of Sciences, which holds a 13.75% minority stake in exchange for priority licensing rights to institute-developed technologies and access to shared research resources. The company is also a partner in the OP JAK Multiomics Project consortium (with Charles University, Institute of Biotechnology, and GeneSpector at BIOCEV) and participates in the TAČR National Center of Competence (project TN02000132). Its customer base is globally distributed across academic core labs and biotech companies, evidenced by publications from institutions such as KU Leuven, Cambridge, Pasteur, Michigan, Bordeaux, NRC Canada, Hebrew University, and AXON Neuroscience. Operations are headquartered at Zlonínská 256, Mratín 250 63 (legal address) with production and R&D at Nad Safinou II 365, Vestec 252 50, Czech Republic. AffiPro maintains no disclosed external venture capital or private equity investment and is founder-led, with no public financial disclosures, headcount figures, or revenue figures available in the input data.

Name

Affibinder S.r.o.

Legal name

AffiPro s.r.o.

Website

https://affipro.cz

Company type

Private

Operating status

Operating

Headcount range

1–10 employees

Short description

AffiPro s.r.o. is a Czech biotech spin-off that manufactures proteomics sample-preparation tools — acidic protease columns, the HDX Workstation, and 3D-printed Affinity MALDI plates — sold globally via direct e-commerce to structural mass spectrometry researchers in academic and biotech laboratories.

Ownership category

akta.pro rank

Product category

Proteomics Laboratory Equipment

NAICS

Analytical Laboratory Instrument Manufacturing (334516)

SIC

Laboratory Analytical Instruments (3826)

akta.pro primary industry

Mass Spectrometry & Spectroscopy Instruments (HLAGAIAB)

akta.pro secondary industries

Proteomics & Mass Spectrometry Services (HLAGANAB), Proteomics & Biomarker Analytical Testing (HLAGADAG)

Keywords

• Proteomics sample preparation
• Mass spectrometry columns
• Enzyme columns
• HDX-MS automation
• MALDI sample preparation

Headquarters

HQ city

Vestec

HQ country

Czechia

HQ region

Europe

Offices2 records

Markets served

GTM type

B2B

Offering type

Hardware or Manufacturing

Cost components

Operations, Technology or R&D, Supply Chain, Marketing or Sales, Personnel

Revenue model

1. Enzyme and Glycanase Columns: Sale of protease columns (Pepsin, Rhizopuspepsin, Nepenthesin-1/2, Aspergillopepsin, AnPep), mixed columns, and PNGase rc glycanase columns for on-line protein digestion and deglycosylation in LC-MS workflows.
2. HDX Workstation Systems: Sale of HDX Workstation instruments — automated systems for Hydrogen-Deuterium Exchange Mass Spectrometry.
3. Affinity MALDI Plates: Sale of functionalized MALDI chips for mass spectrometry sample preparation.

Pricing tiers

Go-to-market motion2 records

Distribution channels3 records

Marketing channels5 records

Core offering

AffiPro manufactures and sells specialized enzyme columns (acidic protease columns, glycanase columns) for on-line protein and RNA digestion in LC-MS workflows, an HDX Workstation for automated Hydrogen-Deuterium Exchange Mass Spectrometry, and functionalized Affinity MALDI plates for mass spectrometry sample preparation. The products serve structural proteomics researchers, biopharmaceutical developers, and academic labs performing HDX-MS, antibody characterization, and membrane protein analysis.

Product overview

AffiPro is a laboratory equipment company specializing in proteomics and structural mass spectrometry tools. The product portfolio centers on enzyme columns for on-line protein and RNA digestion (including Pepsin, Rhizopuspepsin, Nepenthesin, Aspergillopepsin, AnPep, and PNGase rc columns), an HDX Workstation for Hydrogen-Deuterium Exchange Mass Spectrometry, and Affinity MALDI plates for sample preparation. The company is a spin-off from the Institute of Microbiology of the Czech Academy of Sciences and is part of the National Center of Competence supported by the Technology Agency of the Czech Republic.

Differentiator

Problem solved

Functional benefit

Products and services

• AffiPro Pepsin Column Protease LC-MS column for on-line protein digestion using immobilized pepsin. Part number AP-PC-001. For structural proteomics and HDX-MS researchers.
• AffiPro Rhizopuspepsin Column Protease LC-MS column using Rhizopuspepsin protease for on-line protein digestion with a distinct cleavage preference. Part number AP-PC-002.
• AffiPro Nepenthesin-1 Column Protease LC-MS column using Nepenthesin-1 protease for on-line protein digestion. Part number AP-PC-003.
• AffiPro Nepenthesin-2 Column Protease LC-MS column using Nepenthesin-2 protease for on-line protein digestion. Part number AP-PC-004.
• AffiPro Aspergillopepsin Column Protease LC-MS column for protein digestion using Aspergillopepsin enzyme with distinct cleavage specificity. Part number AP-PC-005.
• AffiPro Nepenthesin-2/Pepsin Mixed Column Mixed stationary phase column combining Nepenthesin-2 and Pepsin, optimized for antibody and membrane protein characterization. Part number AP-PC-006.
• AffiPro AnPep Column Protease LC-MS column using AnPep (aspartic protease) for on-line protein digestion. Part number AP-PC-007.
• AffiPro AnPep/Pepsin Mixed Column Mixed column with immobilized AnPep and Pepsin in optimized ratio, designed for characterization of antibodies and membrane proteins. Part number AP-PC-008.
• PNGase rc Column Glycanase LC-MS column for removing complex N-linked glycans from proteins, enabling streamlined deglycosylation and glycan analysis. Part number AP-GC-001.
• RNase T1 Column Immobilized RNase T1 column for online RNA digestion, compatible with LC-MS in a single workflow, with proven RNase T1 specificity and good reproducibility.
• HDX Workstation State-of-the-art automation system for Hydrogen-Deuterium Exchange Mass Spectrometry (HDX-MS), designed for precision, efficiency, and reliability in structural mass spectrometry workflows. For HDX-MS researchers in academia and biotech.
• Affinity MALDI Plates Functionalized MALDI chips prepared by 3D printing combined with ambient ion landing, representing next-generation sample preparation technology for mass spectrometry. For proteomics and mass spectrometry sample preparation labs.

Quantifiable outcome

• Full protein sequence coverage with overlapping peptides achieved through smart choice of protease column combinations, denaturant agents, and flow rate optimization

Named customers16 records

Segments3 records

Ideal customer profiles3 records

Technology focussed Yes

API detail

Has API

No

API docs

API detail

Core technology

AI maturity

App detail

Feature5 records

Partnerships

Three partnerships are on record, tiered core.

• Institute of Microbiology of the Czech Academy of SciencescoreStrategic or Co-development Partner · 1 May 2025The Institute of Microbiology of the Czech Academy of Sciences became a minority shareholder of AffiPro s.r.o. in May 2025, acquiring 13.75% ownership. This joint venture formalized AffiPro as an official spin-off of the Institute, granting AffiPro access to shared resources and priority licensing of technologies developed at the Institute. The partnership aims to commercialize unique technologies from the Institute while helping AffiPro intensify development of new tools for the biotech industry and structural mass spectrometry.
• Multiomics Project Consortium (under OP JAK)coreStrategic or Co-development Partner · 1 January 2025AffiPro is a partner in the Multiomics Project under the Jan Amos Komenský Operational Programme (OP JAK) of the Czech Ministry of Education, Youth and Sports. The project serves as a platform for identifying biological correlates of diseases and developing new diagnostic, preventive, and therapeutic approaches. Consortium members include First Faculty of Medicine at Charles University, Institute of Biotechnology of the Czech Academy of Sciences, GeneSpector s.r.o., and AffiPro s.r.o., all operating at the BIOCEV research center.
• Technology Agency of the Czech Republic (TAČR) — National Center of CompetencecoreStrategic or Co-development PartnerAffiPro is part of the National Center of Competence, supported by the Technology Agency of the Czech Republic under project TN02000132. This government-backed initiative supports research and development in strategic technology areas, providing funding and institutional legitimacy to AffiPro's R&D activities.

Scale indicators2 records

Recent moves7 records

Expansion highlights5 records

Broad incumbents

• Shimadzu: Shimadzu provides mass spectrometry and chromatography systems including MALDI-TOF and HPLC platforms, placing it as a broad incumbent against AffiPro's MALDI plates and HDX-MS consumables.
• Waters Corporation: Waters is a leading mass spectrometry and chromatography provider with bundled HDX-MS solutions, columns, and software. AffiPro's HDX Workstation and Waters-fitted column variants position AffiPro as a complementary/acid-protease specialist against Waters' broader HDX-MS ecosystem.
• Bio-Rad Laboratories: Bio-Rad provides protein analysis and proteomics tools including chromatography consumables and protein characterization reagents, positioning it as a broad incumbent in AffiPro's adjacent proteomics sample preparation market.
• Bruker: Bruker offers HDX-MS-enabled mass spectrometry platforms (e.g., timsTOF, HDX-enabled workflows) and is a major presence at ASMS and HDX-MS conferences, competing with AffiPro's HDX Workstation at the systems level.
• Cytiva: Cytiva (formerly GE Healthcare Life Sciences) supplies chromatography columns, resins, and lab consumables for protein analysis, overlapping with AffiPro's immobilized protease column offerings at the workflow level.
• Agilent Technologies: Agilent provides mass spectrometry and chromatography solutions including columns and consumables for proteomics workflows, making it a broad incumbent against AffiPro's specialized enzyme columns and HDX-MS tools.
• Thermo Fisher Scientific: Thermo Fisher is the largest mass spectrometry and proteomics tools vendor, offering HPLC columns, proteases, and HDX-MS workflows that overlap with AffiPro's enzyme columns, MALDI plates, and HDX instrumentation.
• Sigma-Aldrich (Merck Life Science): Merck/Sigma-Aldrich supplies proteases, chromatography reagents, and mass spectrometry standards used in HDX-MS workflows, giving it broad incumbent reach across the proteomics sample preparation value chain AffiPro addresses.

Direct peers

• Promega: Promega supplies proteomics reagents, enzymes, and sample preparation tools that compete with AffiPro's enzyme columns and HDX-MS consumables, serving the same structural biology and antibody characterization markets.
• Trajan Scientific: Trajan Scientific (formerly SGE Analytical Science) manufactures analytical columns and consumables for chromatography and mass spectrometry, including immobilized protease columns for protein digestion — directly competing with AffiPro's enzyme column portfolio.

Market position

Strengths5 records

Weaknesses5 records

Competitive moat5 records

Key risks7 records

Key highlights7 records

Customer concentration

• YouTubeyoutube.com/@AffiPro-HDX
• LinkedInlinkedin.com/company/affipro

Revenue estimate

Valuation estimate

Number of profiles

Profiles1 record

Funding overview

Funding rounds

Investors

Funding detail is available on the Subscription and Enterprise plan.Contact sales →

M&A

Investments

M&A and investment is available on the Subscription and Enterprise plan.Contact sales →

Frequently asked questions about Affibinder S.r.o.