What Biobide does

Biobide (legal entity BBD BIOPHENIX, S.L.) is a Contract Research Organization (CRO) headquartered at the Miramon Technology Park in San Sebastian, Spain, with a US sales subsidiary (BBD Biophenix USA LLC) in Boston, MA. Founded in 2005, the company provides preclinical toxicology and efficacy testing services using the zebrafish (Danio rerio) as an alternative vertebrate model, serving pharmaceutical, biotechnology, chemical, cosmetic, and nutraceutical clients, alongside patient advocacy organizations such as Duchenne Parent Project España and ApoyoDravet for rare disease research.

The company's core technology is a zebrafish-based preclinical platform comprising a facility housing more than 80,000 adult zebrafish across 35+ lines (some generated in-house), an Automated High Content Screening (HCS) platform operating in 96-well plate in vivo format, and a portfolio of more than 25 validated assays. These include general toxicity (Acutetox, Teratotox), specific organ toxicity (Cardiotox, Hepatotox, Neurotox, Ototox, Immunotox, Multitox, Kidney Toxicity, Muscle Toxicity, ALS Models, Sperm Quality), ecotoxicology under OECD guidelines (EASZY Estrogen Pathway, Daphnia Immobilization, Algae Growth Inhibition, Thyroid Disruption), efficacy (Angiogenesis Inhibition, Antioxidation, Regeneration, Melanin Quantification, Neurodegenerative/Rare Diseases), disease model generation via CRISPR, morpholinos, and transgenic techniques, and target validation services. Selected studies are conducted under Good Laboratory Practice (GLP) compliance for regulatory-grade aquatic ecotoxicology submissions.

Biobide generates revenue through project-based contracts with enterprise clients. Pricing is quote-based and project-specific, dependent on assay complexity, GLP requirements, and throughput, and is not publicly disclosed. Go-to-market is sales-led via business development representatives in Europe and a dedicated US team in Boston. The company has been a wholly-owned subsidiary of Biatgroup (formerly Bionaturis Group, MaB: BST) since 2014 and operates with 11-50 employees.

Name

Biobide

Legal name

BBD BIOPHENIX, S.L.

Website

https://biobide.com

Company type

Private

Founded year

2005

Operating status

Operating

Headcount range

11–50 employees

Short description

Biobide is a Spain-based Contract Research Organization that provides preclinical toxicology, ecotoxicology, and efficacy testing services using zebrafish models for pharmaceutical, biotechnology, chemical, cosmetic, and nutraceutical clients, with a US sales subsidiary in Boston.

Ownership category

akta.pro rank

Product category

Preclinical Research Services

NAICS

Research and Development in Biotechnology (except Nanobiotechnology) (541714), Scientific Research and Development Services (5417)

SIC

Services-Commercial Physical & Biological Research (8731), Services-Testing Laboratories (8734)

akta.pro primary industry

Toxicology & Safety Assessment CROs (GLP) (HLAGAAAC)

akta.pro secondary industry

Preclinical CROs (Discovery/Pharmacology/DMPK) (HLAGAAAB)

Keywords

• Zebrafish preclinical testing
• Contract research organization
• Ecotoxicology GLP studies
• Drug discovery assays
• Toxicology screening services

Headquarters

HQ city

Donostia-san Sebastián

HQ country

Spain

HQ region

Europe

Offices1 record

Markets served

GTM type

B2B

Offering type

Services

Cost components

Personnel, Operations, Infrastructure, Technology or R&D, Marketing or Sales

Revenue model

1. Contract Research Services: Biobide operates as a Contract Research Organization (CRO) providing preclinical toxicity and efficacy testing services using zebrafish models. Services include general toxicity assays, specific toxicity assays, ecotoxicology assays, efficacy assays, disease model generation, and target validation services. Revenue is generated through project-based contracts with pharmaceutical, biotech, chemical, cosmetic, and nutraceutical companies.

Go-to-market motion1 record

Distribution channels2 records

Marketing channels6 records

Core offering

Biobide is a Contract Research Organization (CRO) that sells zebrafish-based preclinical testing services to pharmaceutical, biotech, chemical, cosmetic, and nutraceutical companies. Offerings include general and specific toxicity assays, aquatic ecotoxicology studies, efficacy assays, custom disease model generation, and target validation services, executed under Good Laboratory Practice (GLP) standards via an automated High Content Screening platform.

Product overview

Biobide is a Contract Research Organization (CRO) specializing in zebrafish-based preclinical testing services. The company offers a comprehensive portfolio of validated toxicity and efficacy assays, disease model generation, and target validation services organized into five main categories: General-Toxicity-Assays (Acutetox, Teratotox), Specific-Toxicity-Assays (Cardiotox, Hepatotox, Neurotox, Ototox, Immunotox, Multitox, Kidney Toxicity, Muscle Toxicity, ALS Models, Sperm Quality), Ecotox-Assays (FET Test, Daphnia Immobilization, Algae Growth Inhibition, EASZY Estrogen Pathway, Thyroid Disruption), Efficacy-Assays (Angiogenesis Inhibition, Antioxidation, Regeneration, Melanin Quantification, Neurodegenerative/Rare Diseases), and Disease-Model-Generation/Target-Validation services. All services can be conducted under GLP compliance for regulatory submissions. The company also provides an Automated High Content Screening (HCS) platform for high-throughput compound screening using fluorescent zebrafish lines.

Differentiator

Problem solved

Functional benefit

Products and services

• Acutetox Assay (General Toxicity) Rapid preliminary acute toxicity testing service using zebrafish embryos to provide early safety assessment of compounds for pharmaceutical, chemical, cosmetic, and nutraceutical clients.
• Teratotox Assay Teratogenicity testing service that evaluates developmental toxicity and malformations across multiple morphological endpoints in zebrafish embryos.
• Cardiotox Assay Cardiovascular toxicity screening service leveraging fluorescent zebrafish lines for real-time cardiac assessment of drug candidates and chemicals.
• Hepatotox Assay Hepatotoxicity assessment service using zebrafish liver models to evaluate drug-induced liver injury for pharmaceutical and chemical safety programs.
• Neurotox Assay Neurotoxicity screening service evaluating compound effects on nervous system development and function in zebrafish embryos.
• Ototox Assay Ototoxicity testing service for assessing drug-induced hearing and balance system damage using zebrafish models.
• Immunotox Assay Immunotoxicity assessment service evaluating compound effects on immune system development and function in zebrafish.
• Multitox Assay Comprehensive multi-organ toxicity screening service assessing compound effects across multiple tissue types simultaneously in zebrafish embryos.
• Kidney Toxicity Assay Nephrotoxicity assessment service using zebrafish to evaluate compound effects on kidney development and function.
• Muscle Toxicity Assay Assessment of myotoxicity and muscle development effects using zebrafish disease models.
• Zebrafish Models for Amyotrophic Lateral Sclerosis (ALS) Disease model generation service for amyotrophic lateral sclerosis using zebrafish to study neurodegenerative mechanisms and evaluate candidate therapeutics.
• Sperm Quality Assessment Evaluation of reproductive toxicity through analysis of sperm quality parameters in zebrafish models.
• Zebrafish Sperm Cryopreservation and In Vitro Fertilization Specialized service for cryopreservation of zebrafish sperm and in vitro fertilization techniques to support client research and breeding programs.
• Acutetox Assay - Ecotox Fish embryo acute toxicity test following OECD 236 Guideline for environmental risk assessment of chemicals, targeting chemical and agrochemical registrants.
• Daphnia Immobilization Test Acute immobilization test with Daphnia magna for aquatic toxicity assessment per OECD guidelines, supporting chemical registration studies.
• Microplate Alga Growth Inhibition Test Algal growth inhibition assay for evaluating aquatic toxicity of chemical compounds, supporting ecotoxicology regulatory submissions.
• EASZY Assay (Estrogen Pathway) Estrogen pathway assay for ecotoxicological assessment, evaluating endocrine-disrupting effects of compounds using zebrafish embryos.
• Thyroid Disruption Assay Endocrine disruption assessment targeting the thyroid pathway using zebrafish embryos for chemical and pharmaceutical safety programs.
• Antioxidation Assay Antioxidant capacity evaluation service for cosmetics, nutraceuticals, and pharmaceutical compounds using zebrafish embryos.
• Regeneration Assay Tissue regeneration assessment using zebrafish's natural regenerative capabilities to evaluate therapeutic potential for pharmaceutical clients.
• Melanin Quantification Assay Pigmentation analysis service for evaluating melanogenesis and skin-lightening compound efficacy, used by cosmetic clients.
• Neurodegenerative and Rare Diseases Models Zebrafish-based disease models for studying Alzheimer's, Parkinson's, epilepsy, Dravet syndrome, and other rare diseases for translational research.
• Cancer: Angiogenesis Inhibition Assay Anti-angiogenic efficacy testing service using zebrafish tumor models to evaluate anticancer compounds.
• Disease Model Generation Custom zebrafish disease model generation service using CRISPR, morpholinos, and transgenic techniques tailored to specific client research needs.
• Target Validation Services Gene target identification and validation service using zebrafish models to support early drug discovery and lead selection for pharmaceutical and biotech clients.
• GLP Aquatic Ecotoxicology Studies Good Laboratory Practice (GLP) compliant aquatic ecotoxicology studies for regulatory submission following international guidelines, offered to chemical, agrochemical, and pharmaceutical registrants.

Quantifiable outcome

• Time and cost reduction in drug development through efficient zebrafish screening
• +1 more outcomes

Named customers7 records

Segments5 records

Ideal customer profiles4 records

Technology focussed No

API detail

Has API

No

API docs

API detail

Core technology

AI maturity

App detail

Feature3 records

Partnerships

15 partnerships are on record, tiered minor and core.

• ASEBIOminorOthersSpanish biotechnology association membership providing networking, industry advocacy, and visibility within the Spanish biotech ecosystem.
• BasqueBiominorOthersBasque health cluster association connecting biotechnology companies in the Basque region for collaborative opportunities.
• BiobasqueminorOthersBasque biotechnology organization supporting biotech development and collaboration in the region.
• DIAminorOthersDrug Information Association - global forum for regulated healthcare product discussions and regulatory science.
• ETS (European Teratology Society)minorOthersEuropean scientific society focused on developmental and reproductive toxicology.
• Eufish BiomedminorStrategic or Co-development PartnerEuropean consortium for fish biomedicine research, providing collaborative opportunities in aquatic model organism research.
• HOT (Human Toxicology - OECD)minorOthersHuman toxicology special interest group under the Society of Toxicology focusing on alternative testing methods.
• CILcareminorOthersNanomedicine Spain association for hearing research and ototoxicity expertise.
• SPS (Safety Pharmacology Society)minorOthersGlobal society dedicated to safety pharmacology, supporting regulatory science and best practices.
• SETACminorOthersSociety of Environmental Toxicology and Chemistry - global organization for ecotoxicology and environmental science.
• SOT (Society of Toxicology)minorOthersProfessional society for toxicology researchers and practitioners, providing conference participation and industry networking.
• ZFIN (Zebrafish Information Network)minorOthersUniversity of Oregon-based zebrafish model organism database and community resource.
• ZHA (Zebrafish Husbandry Association)minorOthersInternational organization dedicated to excellence in zebrafish husbandry and facility management standards.
• Hazitek ProjectsminorStrategic or Co-development PartnerBasque government-funded R&D support programs including Hazitek 2025, Daniobit 2023, and other innovation projects supporting zebrafish research development.
• BBD Biophenix USA LLCcoreChannel Partner/ Reseller/ DistributorUS subsidiary of Biobide operating as a sales and business development office in Boston, MA, responsible for North American market engagement and client relationships.

Scale indicators4 records

Recent moves7 records

Expansion highlights6 records

Broad incumbents

• Eurofins Scientific: International testing services group with significant life sciences and preclinical testing capabilities. Comparable broad incumbent providing toxicology and ecotoxicology testing services to overlapping enterprise clients.
• Evotec: Global large-cap CRO with broad preclinical and drug discovery services, including zebrafish-based screening capabilities. Comparable as a broad incumbent in preclinical CRO services targeting the same pharma/biotech customers.
• WuXi AppTec: Global CRO/CDMO offering preclinical, toxicology, and integrated drug development services. Comparable broad incumbent competing for preclinical toxicology and discovery contracts with the same pharma/biotech clients.
• Charles River Laboratories: Large global CRO with extensive preclinical safety/toxicology services and a broad portfolio. Comparable as a broad incumbent competing for the same preclinical safety and toxicology budgets as Biobide.
• Labcorp Early Development: Global CRO offering preclinical, nonclinical safety assessment, and analytical services through its Early Development segment. Comparable broad incumbent in the same preclinical toxicology category as Biobide.

Emerging players

• Inotiv: US-based preclinical CRO focused on nonclinical safety assessment, toxicology, and research models. Comparable emerging-to-mid player competing for the same preclinical toxicology spend as Biobide.
• CILcare: Hearing research CRO using zebrafish ototoxicity models (a partnership referenced in Biobide's alliance list). Comparable emerging player with overlapping zebrafish-based ototoxicity/preclinical service offerings.
• Bionova Scientific: Mid-sized CRO offering preclinical and analytical services for biologics developers. Comparable emerging player in the broader preclinical CRO space serving pharma/biotech customers with overlapping needs.

Direct peers

• ZeClinics: Barcelona-based zebrafish CRO providing preclinical toxicity and efficacy services to pharma and biotech. Direct peer — same zebrafish model, same preclinical CRO offering, with overlapping enterprise pharma customer base.
• Phylonix Pharmaceuticals: US-based zebrafish CRO offering preclinical drug discovery, toxicity, and efficacy testing services using zebrafish models. Direct peer — comparable specialty CRO business model focused exclusively on the zebrafish platform.

Market position

Strengths5 records

Weaknesses5 records

Competitive moat4 records

Key risks6 records

Key highlights7 records

Customer concentration

• Xx.com/Biobide
• YouTubeyoutube.com/channel/UCE979rIc4g09AF2LBr7uoZg
• Facebookfacebook.com/Biobide-1286387971453667
• LinkedInes.linkedin.com/company/biobide

Revenue estimate

Valuation estimate

Number of profiles

Profiles9 records

Subsidiaries1 record

Funding overview

Funding rounds

Investors

Funding detail is available on the Subscription and Enterprise plan.Contact sales →

M&A

Investments

M&A and investment is available on the Subscription and Enterprise plan.Contact sales →

Frequently asked questions about Biobide