Environmental Enterprises USA

What Environmental Enterprises USA does

Environmental Enterprises USA, Inc. is a privately held, NELAP-accredited aquatic toxicity testing laboratory headquartered in Slidell, Louisiana, in continuous operation since April 1987. The company provides regulatory-grade bioassay and analytical testing services to the offshore and onshore oil and gas industry, municipal wastewater utilities, and industrial plants and refineries in the Gulf South region and internationally. Core tests include Whole Effluent Toxicity (WET) assessments using freshwater and saltwater indicator organisms, drilling fluids and produced water toxicity testing, sediment toxicity testing, oil and grease analysis (EPA Method 1664A), Chemically Treated Waters (CTS) testing, NCP compliance testing for oil spill response products, and a 275-day closed-bottle biodegradation test (ISO 11734-1995/EPA Method 1647). Supporting offerings include in-house aquaculture of seven EPA-required test species, GC/MS analysis of crude oil contamination in non-aqueous drilling fluids (EPA Method 1655), NPDES permit compliance data management and Discharge Monitoring Report preparation, and a recently launched retail line selling cultured test organisms to other laboratories.

The company's underlying technology is built around standardized EPA methods executed in a purpose-built 13,500+ square-foot facility featuring a private deep water well, four independent environmental chambers, and gas-powered backup generation. The in-house aquaculture department is technically distinctive: EE USA cultures Americamysis bahia, Artemia salina, Ceriodaphnia dubia, Daphnia pulex, Leptocheirus plumulosus, Menidia beryllina, and Pimephales promelas internally, providing immediate organism availability and reference-toxicity quality control that reduces supply-chain risk for routine testing. Routine throughput is reported at 40+ pairs of 7-day chronic tests and up to 150 acute toxicity tests per month, with 24-hour turnaround for completion/workover fluid analyses.

EE USA operates a sales-led B2B lab services model with direct account engagement, free scheduled pickup and delivery across approximately eleven Gulf South shore bases, and 24/7 after-hours sample acceptance for an additional fee. Pricing is quote-based and not publicly disclosed; revenue is generated primarily through per-test professional service fees, supplemented by ancillary organism sales, rush service premiums, and recurring compliance data management contracts. Customers exceed 100, including offshore and onshore operators in the Gulf of Mexico, U.S. municipalities, and chemical and refining plants, with samples additionally received from South America, Europe, the Middle East, and Asia.

Name

Environmental Enterprises USA

Legal name

Environmental Enterprises USA, Inc.

Website

https://eeusa.com

Company type

Private

Founded year

1987

Operating status

Operating

Headcount range

11–50 employees

Short description

Environmental Enterprises USA is a privately held, NELAP-accredited aquatic toxicity testing laboratory in Slidell, Louisiana, providing EPA-standardized bioassay and analytical testing to oil and gas operators, municipalities, and industrial plants primarily across the Gulf South region and internationally.

Ownership category

akta.pro rank

Product category

Environmental / Aquatic Toxicity Testing Services

NAICS

Testing Laboratories and Services (54138), Environmental Consulting Services (541620)

SIC

Services-Testing Laboratories (8734), Services-Commercial Physical & Biological Research (8731)

akta.pro primary industry

Wastewater & Industrial Effluent Monitoring (NPDES/Permit Compliance) (EUAHACAD)

akta.pro secondary industry

Laboratory Testing & Analytical Services (in-house/contract labs; microbiology, metals, organics) (EUANAJAB)

Keywords

• Aquatic toxicity testing
• Environmental compliance services
• Whole effluent toxicity
• NPDES permit testing
• Oil and gas laboratory

Headquarters

HQ city

Slidell

HQ country

United States

HQ region

North America

Offices1 record

Markets served

GTM type

B2B

Offering type

Services

Cost components

Personnel, Operations, Infrastructure, Technology or R&D, Supply Chain

Revenue model

1. Aquatic Toxicity Testing Services: The company generates revenue through providing comprehensive aquatic toxicity testing services including freshwater testing, saltwater testing, produced water toxicity, drilling fluids testing, sediment toxicity, NCP testing, CTS toxicity testing, and biodegradation testing. They charge for these laboratory analysis services based on the type and number of tests performed.
2. Test Organisms Sales: After decades of culturing quality test organisms for internal use, EE USA entered the test organism supply market. They sell freshwater and saltwater organisms cultured in-house, with standard reference toxicity testing completed routinely. Contact: David L. Daniel for pricing.
3. Data Management and Compliance Services: EE USA provides environmental compliance data management services including permit compliance data management for EPA, LA DEQ, and Texas RRC; permit applications; non-compliance reports; electronic reporting setup; monthly compliance review; and Discharge Monitoring Report preparation.
4. Oil and Grease Analysis: Oil and grease analysis services including Produced Water HEM (EPA Method 1664A), Total Petroleum Hydrocarbons, and completion fluids analysis. Standard turnaround is 24 hours for completion/workover fluids and 5-7 business days for produced water, with 24-hour RUSH service available.

Pricing tiers

Go-to-market motion1 record

Distribution channels3 records

Marketing channels2 records

Core offering

Environmental Enterprises USA operates a NELAP-accredited aquatic toxicity testing laboratory providing EPA-standardized Whole Effluent Toxicity (WET) tests, GC/MS analytical testing, oil and grease analysis, sediment toxicity testing, drilling fluids and produced water toxicity testing, NCP and CTS toxicity testing, and 275-day biodegradation testing for oil and gas operators, municipalities, and industrial plants. The company also offers in-house cultured test organism supply, environmental compliance data management including NPDES Discharge Monitoring Report preparation, and free pickup/delivery sampling logistics across Gulf South shore bases.

Product overview

Environmental Enterprises USA is a NELAP-accredited aquatic toxicity testing laboratory offering a comprehensive suite of testing services for the oil and gas industry, municipalities, and industrial plants. The core product portfolio includes freshwater and saltwater toxicity testing services using EPA-approved methods, with specialized offerings for drilling fluids, produced water, sediment, oil and grease analysis, NCP compliance testing, CTS toxicity, GC/MS analysis, and biodegradation testing. Supporting services include in-house aquaculture for test organisms, a newly launched organisms-for-sale program, environmental compliance data management, and sampling logistics. The company operates over 13,500 square feet of laboratory space and provides services to clients throughout the United States and internationally.

Differentiator

Problem solved

Functional benefit

Products and services

• Freshwater Toxicity Testing Whole Effluent Toxicity (WET) tests using EPA-method 2002 (Ceriodaphnia dubia), 2021 (Daphnia pulex), and 2000 (Pimephales promelas) for 24, 48, and 96-hour definitive acute and chronic testing of effluent discharged into surface water, for wastewater treatment plants, chemical plants, refineries, and product manufacturers.
• Saltwater Toxicity Testing Acute and chronic toxicity testing for marine and estuarine organisms using Americamysis bahia (mysid shrimp) and Menidia beryllina (inland silverside minnow) following EPA methods, for oil and gas, industrial, and municipal clients discharging into marine and estuarine waters.
• Produced Water Toxicity Testing 7-day chronic toxicity tests using Americamysis bahia and Menidia beryllina for evaluating the toxicity of produced water from oil and gas operations, with standard 5-7 business day turnaround and 24-hour RUSH service available.
• Drilling Fluids Toxicity Testing Toxicity testing for water-based and synthetic-based drilling fluids using suspended particulate phase (SPP) tests with Americamysis bahia, Leptocheirus plumulosus, and Artemia salina, including sediment toxicity tests and GC/MS analysis for crude oil contamination.
• Sediment Toxicity Testing Toxicity tests evaluating chemicals accumulated in sediment and their effects on benthic organisms including Leptocheirus plumulosus, Chironomus dilutus, Corophium volutator, and Ampelisca abdita.
• Oil and Grease Analysis Analysis of produced water and completion fluids using EPA Method 1664A for Hexane Extractable Material (HEM) and Total Petroleum Hydrocarbons (TPH) with silica gel treatment, with 24-hour standard turnaround for completion/workover fluids and 5-7 business day turnaround for produced water with 24-hour RUSH service.
• NCP Product Schedule Toxicity Testing Toxicity testing required for products to be listed on the National Contingency Plan (NCP) Product Schedule for oil spill response, involving exposure of Menidia beryllina and Americamysis bahia to products, No. 2 fuel oil, and mixtures following NCP standard dispersant toxicity test methods.
• CTS (Chemically Treated Saltwater/Freshwater) Toxicity Testing Chemically Treated Saltwater/Freshwater toxicity testing for EPA Region VI and Region IV using Americamysis bahia and Menidia beryllina with 48-hour NOEC statistical analysis.
• GC/MS Analysis (EPA Method 1655) Gas Chromatography/Mass Spectrometry analysis for determination of crude oil contamination in non-aqueous drilling fluids using EPA Method 1655, with 1-2 business day standard turnaround.
• 275-Day Closed Bottle Biodegradation Testing ISO 11734-1995 / EPA Method 1647 biodegradation testing determining degradation of base fluids by monitoring gas production due to microbial degradation in marine sediment.
• Aquaculture Services (In-House Test Organism Culturing) In-house culturing of freshwater and saltwater test organisms including Americamysis bahia, Artemia salina, Ceriodaphnia dubia, Daphnia pulex, Leptocheirus plumulosus, Menidia beryllina, and Pimephales promelas for immediate availability and quality control in support of all toxicity testing services.
• Test Organisms for Sale Freshwater and saltwater test organisms cultured in-house sold to external laboratories and research institutions, with standard reference toxicity testing completed routinely to ensure consistent high quality; pricing is quote-based via David L. Daniel.
• Environmental Compliance and Data Management Services Offshore water permit compliance data management and reporting for EPA, Louisiana DEQ, and Texas RRC including permit applications, non-compliance reports, electronic Discharge Monitoring Report preparation, monthly compliance review, and consulting services.
• Sampling Logistics Services Sample kit provision with containers, sampling instructions, and chain-of-custody forms; coordinated free pickup and delivery to Gulf South shore bases (Patterson, Houma, Grand Isle, Fourchon, Galliano, Venice, Boothville, Cameron, ICY, Mouton Cove, Abbeville); 24/7 after-hours sample acceptance; water quality sample collection for Sanitary Wastewater general permit.

Quantifiable outcome

• Routinely complete 40+ pairs of 7-day chronic tests and up to 150 acute toxicity tests per month
• +3 more outcomes

Named customers3 records

Segments3 records

Ideal customer profiles4 records

Technology focussed No

API detail

Has API

No

API docs

API detail

Core technology

AI maturity

App detail

Feature4 records

Scale indicators5 records

Recent moves9 records

Expansion highlights5 records

Direct peers

• Pace Analytical Services: Pace is one of the largest U.S.-focused environmental testing lab networks, offering NPDES, WET, and effluent toxicity testing for industrial and municipal clients. It is a direct competitor for the same regulated aquatic toxicology work EE USA performs in the Gulf South.
• Nautilus Environmental: Nautilus is a specialty aquatic toxicology laboratory offering freshwater, saltwater, and sediment toxicity testing under EPA and NELAP-equivalent protocols. A directly comparable niche specialist competing for WET and NPDES work.
• Smithers (Environmental Risk Sciences): Smithers' environmental practice performs ecotoxicology, biodegradation, and effluent testing for chemical, oil and gas, and consumer product clients. Directly comparable on biodegradation and whole-effluent toxicity testing.
• Pacific EcoRisk Laboratories: Pacific EcoRisk is an aquatic toxicology specialty lab providing freshwater, marine, and sediment toxicity testing to industrial and municipal clients. Directly comparable to EE USA in scope, methods, and customer profile.
• ALS Global (ALS Environmental): ALS operates a global environmental testing network with strong ecotoxicology and NPDES capabilities, serving oil and gas, municipal, and industrial end markets. A direct competitor for EE USA's core service lines.

Broad incumbents

• Intertek Oil, Gas and Environmental Services: Intertek provides laboratory and field testing across energy and environmental verticals, including produced water and drilling fluids analysis. Comparable to EE USA on oil and gas environmental testing, with a broader portfolio.
• Bureau Veritas Environment & Sustainability: Bureau Veritas provides environmental testing and compliance services globally, including water quality and ecotoxicity testing for oil and gas and industrial clients. A broad incumbent peer competing for similar NPDES and effluent testing work.
• SGS Environment, Health and Safety: SGS is a global testing, inspection, and certification company with a substantial environmental laboratory business serving oil and gas, municipal, and industrial clients. Overlaps with EE USA in NPDES, effluent, and ecotoxicology testing categories.
• Eurofins Environment Testing: Eurofins operates one of the world's largest environmental testing networks, including ecotoxicology and NPDES services. It is comparable to EE USA as a competing aquatic toxicity and wastewater testing provider, but at vastly greater scale and geographic reach.

Regional players

• Premier Laboratory: Premier Laboratory is a Gulf-region environmental testing laboratory offering aquatic toxicity, NPDES, and oil and gas analytical services. A regional competitor with overlapping client base in the Gulf South.

Market position

Strengths5 records

Weaknesses5 records

Competitive moat4 records

Key risks6 records

Key highlights7 records

Customer concentration

• LinkedInlinkedin.com/company/environmental-enterprises-usa

Compliance2 records

Revenue estimate

Valuation estimate

Number of profiles

Profiles4 records

Funding overview

Funding rounds

Investors

Funding detail is available on the Subscription and Enterprise plan.Contact sales →

M&A

Investments

M&A and investment is available on the Subscription and Enterprise plan.Contact sales →

Frequently asked questions about Environmental Enterprises USA