ProTren Probiotics International

What ProTren Probiotics International does

ProTren Probiotics International is a precision probiotic supplement manufacturer founded in 1980 (per company history firmographics record states 1982) by Natasha Trenev and headquartered at 3105 Willow Lane, Westlake Village, California. The company produces a portfolio of professional-grade single-strain and combination probiotic supplements marketed exclusively through licensed healthcare practitioners and their patients. The product line is anchored by the Maximum Strength Series (Trenev Trio 3-in-1 oil-matrix system and the recently launched Trenev Duo), the Single Strength Series (Superdophilus, Bifidonate, Bulgaricum), the Life Start line for infants and sensitive-GI consumers, convenience formats (Gastro-pH wafers, Healthy Tummy, Yogurt Starter), Petbiotics for companion animals, and specialized Autism GI Support regimens with bundled protocols.

The company's core differentiator is the proprietary Trenev Process, a trade-secret oil-matrix delivery system that micro-enrobes probiotic bacteria in sunflower oil and vitamin E to keep strains separated and protected from gastric acid, with near-100% survivability validated at pH 1.8 for 60-90 minutes by an independent European institute. Manufacturing is fully vertical at the Westlake Village facility, spanning culturing through bottling under temperature and humidity control, with thermally controlled shipping (overnight or second-day air only) and a 100% potency guarantee through printed expiration date. MicroPure Technology adds genetic strain identification and contamination testing. Vertical integration and in-house quality control support pharmaceutical-grade positioning in a category where most competitors rely on contract manufacturing.

The business model is a hybrid of wholesale-to-practitioners, direct e-commerce to referred patients, and affiliate-driven top-of-funnel acquisition. Retail pricing spans $21.95-$189.95 for individual products and up to $1,059.50 for 3-month bundled regimens; a $12.95 packaging/handling fee plus a minimum $38 order threshold are applied; wholesale orders under $100 carry an $8 surcharge. Healthcare practitioners access wholesale or courtesy accounts (in-store inventory or drop ship), patients can enroll in Subscribe & Save recurring orders, and an affiliate program offers 10-15% tiered commissions. The company is a private LLC, entirely founder-owned, with no external institutional capital, no M&A history, and headcount in the 11-50 range.

Name

ProTren Probiotics International

Legal name

ProTren Probiotics International, LLC

Website

https://protren.com

Company type

Private

Founded year

1980

Operating status

Operating

Headcount range

11–50 employees

Short description

ProTren Probiotics International is a Westlake Village, California-based manufacturer of precision probiotic supplements sold exclusively through licensed healthcare practitioners and their patients, leveraging a proprietary oil-matrix delivery technology (Trenev Process) to ensure probiotic survivability through stomach acid.

Ownership category

akta.pro rank

Product category

Probiotic Supplements

NAICS

Pharmaceutical Preparation Manufacturing (325412)

SIC

Pharmaceutical Preparations (2834)

akta.pro primary industry

Probiotics & Digestive Health Supplements (CRADAGAF)

akta.pro secondary industries

Probiotics (Strains, Spore-Formers, Synbiotic Components) (AFAJAKAE), Probiotic, Prebiotic & Gut-Health Foods Manufacturing (Fermented Functional Foods, Synbiotics) (AFAKAJAH)

Keywords

• Precision probiotic supplements
• Practitioner-grade probiotics
• Oil-matrix probiotic delivery
• Pharmaceutical grade supplements
• Professional dietary supplements

Headquarters

HQ city

Westlake Village

HQ country

United States

HQ region

North America

Offices2 records

Markets served

GTM type

B2B

Offering type

Hardware or Manufacturing

Cost components

Supply Chain, Personnel, Operations, Technology or R&D, Marketing or Sales

Revenue model

1. Direct Product Sales: ProTren generates revenue through direct sales of precision probiotic supplements via e-commerce website and wholesale accounts for healthcare practitioners. Products include capsules, powders, and wafers in various sizes with retail prices ranging from $21.95 to $189.95 for individual products, and bundled regimens from $72.95 to $1,059.50.
2. Affiliate Program Commissions: Affiliate partners earn 10% or higher commission on all sales, with 15% commission for affiliates achieving $500+ monthly sales volume. Average order value is approximately $140.
3. Wholesale Practitioner Accounts: Healthcare practitioners can open wholesale or courtesy accounts to purchase products at discounted rates for resale or patient distribution. The company offers flexible programs including in-store inventory and drop shipping options.

Pricing tiers

Go-to-market motion3 records

Distribution channels3 records

Marketing channels6 records

Core offering

ProTren Probiotics International manufactures and sells pharmaceutical-grade precision probiotic supplements — including capsules, powders, wafers, and yogurt starters — distributed primarily through licensed healthcare practitioners and direct-to-consumer e-commerce for referred patients. Core products include the Trenev Trio and Trenev Duo multi-strain oil-matrix systems and single-strain Original Strength Series targeted at specific GI-tract regions, supported by proprietary Trenev Process oil-matrix delivery and MicroPure Technology for guaranteed strain identification and potency.

Product overview

ProTren Probiotics International is a precision probiotic supplement company offering a comprehensive portfolio of professional-grade probiotic products sold exclusively through licensed healthcare professionals. The portfolio centers on the Trenev Trio (3-in-1 maximum strength oil-matrix system) and Trenev Duo (2-in-1 colon health formula) as core products, supported by the single-strain Original Strength Series (Superdophilus for small intestine, Bifidonate for large intestine, Bulgaricum for transient flora support). The Life Start line addresses infant and sensitive-GI needs with dairy, goat milk, and dairy-free options. Additional offerings include convenience formats (Gastro-pH chewable wafers, Healthy Tummy diet wafers, Yogurt Starter), Petbiotics for companion animals, and specialized Autism GI Support protocols with bundled regimens. The company differentiates through its proprietary Trenev Process oil-matrix delivery technology that protects probiotic strains through gastric passage.

Differentiator

Problem solved

Functional benefit

Products and services

• Trenev Trio Oil Matrix A maximum-strength 3-in-1 probiotic oil-matrix system that combines three super strains (Lactobacillus acidophilus NAS, Bifidobacterium bifidum Malyoth, Lactobacillus bulgaricus LB-51) in the proprietary Trenev Process oil-matrix delivery to support small intestine, large intestine, and transient flora health. Offered in 15, 30, 60, and 90 capsule bottles for healthcare practitioners and their patients.
• Trenev Duo A 2-in-1 oil-matrix probiotic system containing B. infantis NLS super strain and B. longum NBL super strain, formulated for bifidobacterial renewal and colon health support. Delivered in 90-capsule bottles.
• Superdophilus A single-strain probiotic containing the Lactobacillus acidophilus NAS super strain (dairy free) or DDS-1 super strain (dairy), targeted at supporting small intestine health. Available in 1.75 oz powder and 60-capsule formats.
• Bifidonate A single-strain probiotic containing Bifidobacterium bifidum Malyoth super strain, formulated to support large intestine and colon health. Available in dairy-free powder and dairy powder and capsule formats.
• Bulgaricum A single-strain transient probiotic containing Lactobacillus bulgaricus LB-51 super strain, formulated to travel through the digestive tract supporting digestion of carbohydrates and proteins. Sold in 60-capsule dairy-free bottles.
• Life Start Pro A dairy-free 1.25 oz powder containing Bifidobacterium infantis NLS super strain, formulated for infants, expecting/nursing mothers, adults, and individuals with sensitive GI tracts.
• Life Start A dairy-based powder containing Bifidobacterium infantis NLS super strain, formulated for infants, toddlers, and expecting mothers with probiotic requirements.
• Life Start 2 A goat-milk-based powder and capsule product containing Bifidobacterium infantis NLS super strain, formulated for those with cow's milk sensitivity.
• Gastro-pH Strawberry-flavored chewable wafers containing Lactobacillus bulgaricus LB-51, designed for convenient on-the-go occasional indigestion relief.
• Healthy Tummy French vanilla ice-cream-flavored chewable wafers containing Lactobacillus bulgaricus LB-51, positioned as a low-calorie probiotic treat for dieters.
• Yogurt Starter Bulgarian-style 1.75 oz yogurt starter powder containing Lactobacillus bulgaricus LB-51 and Streptococcus thermophilus BC 122, designed to make up to 30 quarts of probiotic yogurt at home.
• Petbiotics An animal probiotic line including CanineDophilus for dogs, FelineDophilus for cats, and Gastro Vegi-Dophilus powder, formulated to support digestive health and healthy intestinal flora in pets and birds.
• Autism Probiotic Nutritional Support Regimens Four bundled probiotic protocols — Gentle, Moderate, Aggressive, and Aggressive Layering — specifically designed for autistic children, available in 6-week and 3-month supply options.
• Autism Observational Study Regimen A 12-week progressive probiotic regimen designed for children with autism spectrum disorder, with phased dosing that progresses from Life Start 2 to Trenev Trio across the study period.

Quantifiable outcome

• Virtually 100% survivability in stomach acid for over one hour at pH 1.8 (validated by independent European institute testing)
• +2 more outcomes

Named customers6 records

Segments4 records

Ideal customer profiles2 records

Technology focussed No

API detail

Has API

No

API docs

API detail

Core technology

AI maturity

App detail

Feature5 records

Scale indicators8 records

Recent moves6 records

Expansion highlights6 records

Broad incumbents

• Culturelle: Mass-market probiotic brand owned by i-Health (Ferrosan/Chr. Hansen), featuring Lactobacillus rhamnosus GG. Comparable in probiotic focus and strain-led positioning, though distributed primarily through retail rather than practitioner channels.
• Garden of Life: Major premium probiotic and supplement brand owned by Nestlé Health Science, offering practitioner and consumer-grade probiotics. Comparable as a probiotic-focused supplement company serving overlapping gut-health demand, though broader in portfolio and far larger in scale.
• Jarrow Formulas: Broad-line supplement manufacturer with a meaningful probiotic portfolio including shelf-stable spore-based and multi-strain products. Comparable in probiotic manufacturing and multi-channel supplement distribution, though broader in scope.
• Renew Life: Digestive health supplement brand with a strong probiotic portfolio targeting digestive wellness. Comparable probiotic focus and digestive health positioning, though distributed through retail and DTC rather than practitioner-only.

Direct peers

• Metagenics: Practitioner-only nutritional supplement company serving integrative and functional medicine clinicians. Highly comparable distribution model and clinical-endorsement-driven GTM motion, with broader supplement portfolio including probiotics.
• Pure Encapsulations: Practitioner-only hypoallergenic supplement brand owned by Atrium Innovations/Nestlé Health Science. Comparable clinical-grade, practitioner-channel positioning with significant probiotic SKUs competing in the same integrative-medicine category.
• Integrative Therapeutics: Professional-grade supplement brand sold exclusively through healthcare practitioners. Direct peer in the practitioner-channel probiotic and GI-health category with overlapping naturopathic and integrative medicine customer base.
• Thorne Research: Practitioner-channel nutritional supplement company with comparable premium, practitioner-only distribution model and emphasis on clinical-grade quality. Directly comparable GTM motion and overlap in healthcare professional endorsements.
• Klaire Labs: Practitioner-only probiotic and supplement brand (part of ProThera) emphasizing hypoallergenic, clinical-grade formulations. Direct competitor in the practitioner probiotic channel with overlapping healthcare professional customer base.

Emerging players

• Seed Health: Venture-backed synbiotic and probiotic subscription brand targeting consumers with clinically validated formulations. Comparable in clinically validated probiotic positioning but uses a modern DTC subscription model rather than practitioner channel.

Market position

Strengths5 records

Weaknesses5 records

Competitive moat4 records

Key risks6 records

Key highlights7 records

Customer concentration

• LinkedInlinkedin.com/company/protren-inc

Revenue estimate

Valuation estimate

Number of profiles

Profiles2 records

Funding overview

Funding rounds

Investors

Funding detail is available on the Subscription and Enterprise plan.Contact sales →

M&A

Investments

M&A and investment is available on the Subscription and Enterprise plan.Contact sales →

Frequently asked questions about ProTren Probiotics International