PerfuseCell

What PerfuseCell does

PerfuseCell-Cronus A/S is a Danish privately-held manufacturer of upstream single-use perfusion bioreactors for mammalian cell cultivation, serving pharmaceutical process development and small-scale biologics manufacturing customers globally. Operating from Herlev, Denmark as part of the Stobbe Group alongside four sister companies (CerCell, Cronus-PCS, PumpCell, ProfileCell), the firm designs pre-assembled Perfusion-Single-Use-Bioreactors (P-SUBs) built around patented LASER-guided diaphragm pump technology (CerPhragm family — Thalia for ATF and Clio for TFF), integrated SepraPor Hollow-Fiber-Filters from Meissner, and a proprietary Clotho Drive Unit software platform with OPC-UA connectivity to Lucullus and DeltaV. Product lines include CellMembra (PTF/TFF-Integrated Series 30) and CellRetention (ATF-SUBmerged Series 31 and traditional/DIY kit variants), with working volumes from 80 ml to 13 liters enabling cell densities of 75-100 million cells/ml for mAb expression. The company monetizes primarily through direct enterprise hardware sales (P-SUBs, diaphragm pumps, drive units, accessories) on 30-day payment terms with ex-works delivery, supplemented by customized product development services where IP becomes property of the company. Go-to-market relies on direct sales via [email protected], technical trade shows (BPI, ESACT, InterPhex, Dechema, Single-Use Events), newsletters, and a documentation-rich website serving R&D scientists in pharma, biotech, and academia. Notable enterprise validation includes a custom CellMembra-300-DASbox developed for Merck; named native integrations with Sartorius DASbox, Eppendorf Modular, Finesse Smart PCS, and Hamilton/sensor partners anchor the product into existing process control ecosystems.

Name

PerfuseCell

Legal name

PerfuseCell-Cronus A/S

Website

https://perfusecell.com

Company type

Private

Founded year

2008

Operating status

Operating

Headcount range

1–10 employees

Short description

PerfuseCell develops, manufactures, and supplies single-use perfusion bioreactors and LASER-guided diaphragm pumps for mammalian cell cultivation, serving pharmaceutical, biotech, and academic process development customers globally from Herlev, Denmark.

Ownership category

akta.pro rank

Product category

Single-Use Bioreactors / Bioprocess Equipment

NAICS

Medical Equipment and Supplies Manufacturing (3391), Measuring, Dispensing, and Other Pumping Equipment Manufacturing (333914), Research and Development in Biotechnology (except Nanobiotechnology) (541714)

SIC

Surgical & Medical Instruments & Apparatus (3841)

akta.pro primary industry

Upstream Bioprocessing Systems & Equipment (cell culture/fermentation, bioreactors, feeds/media) (HLAAAJAA)

akta.pro secondary industries

Cell Culture & Bioprocess Lab Equipment (Bioreactors, Single-Use Systems, Controllers) (HLAGAIAG), Single-Use Technologies (SUT) & Disposable Fluid Management (bags, assemblies, connectors) (HLAAAJAC)

Keywords

• Single-use bioreactors
• Perfusion bioreactor systems
• Diaphragm pumps
• Cell culture equipment
• Bioprocess hardware

Headquarters

HQ city

Herlev

HQ country

Denmark

HQ region

Europe

Offices1 record

Markets served

GTM type

B2B

Offering type

Hardware or Manufacturing

Cost components

Technology or R&D, Personnel, Supply Chain, Operations, Marketing or Sales, Infrastructure

Revenue model

1. Hardware Product Sales: Sale of perfusion bioreactors (P-SUBs), diaphragm pumps (SUPs), drive units, and accessories. Products include CellMembra, CellRetention, Clotho, Thalia, Clio, and SepraPor HFF systems. Payment terms: 30 days for hardware products. Delivery: Ex works.
2. Customized & Configurable Products: Customization and product design/development services where company develops custom products per customer specifications. Such projects become property of the company.

Go-to-market motion1 record

Distribution channels1 record

Marketing channels4 records

Core offering

PerfuseCell-Cronus A/S develops, manufactures, and supplies single-use Perfusion Bioreactors (P-SUBs) and associated equipment for upstream mammalian cell cultivation in pharmaceutical and biotech process development and small-scale manufacturing. The portfolio centers on two product families: CellMembra (Pulsating-Tangential-Flow, TFF) and CellRetention (Alternating-Tangential-Flow, ATF), both integrating LASER-guided diaphragm Single-Use-Pumps, SepraPor Hollow-Fiber-Filters, drive units, sensors, and process control software.

Product overview

PerfuseCell-Cronus A/S develops, manufactures, and supplies creative single-use equipment for continuous upstream biopharmaceutical processes. The product portfolio centers on two main Perfusion-Single-Use-Bioreactor (P-SUB) product lines: CellMembra (TFF-Integrated Series 30) using Pulsating-Tangential-Flow technology with Clio OneWay-Single-Use-Pumps, and CellRetention (ATF-SUBmerged Series 31) using Alternating-Tangential-Flow technology with Thalia Alternating-Single-Use-Pumps. Both systems integrate SepraPor Hollow-Fiber-Filters and are controlled by Clotho Drive Units featuring LASER-guided diaphragm pump technology. Supporting products include Alagonia vacuum/pressure pumps, Sarpedon reservoirs, comprehensive sensor systems (DO, pH, biomass, temperature), thermal control systems (HSF), and Clotho process software. The company also offers DIY kit series (33, 34) for custom configurations and traditional ATF options (Series 32). All products are designed for mammalian cell cultivation at high cell density (25-100+ million cells/ml) for mAb expression in perfusion or semi-continuous mode. The company belongs to Stobbe Group based in Herlev, Denmark.

Differentiator

Problem solved

Functional benefit

Products and services

• CellMembra TFF-Integrated Series 30 (PTF Perfusion Bioreactors) Perfusion Single-Use-Bioreactor (P-SUB) family using Pulsating-Tangential-Flow (PTF) technology with integrated Clio OneWay-Single-Use-Pump and SepraPor Hollow-Fiber-Filter for mammalian cell cultivation at high cell density. Includes CellMembra-300 BioBLU, CellMembra-500 CellVessel, CellMembra-3 Integrated, and CellMembra-13 Integrated models, with working volumes from 80 ml to 13 liters.
• CellRetention ATF-SUBmerged Series 31 (ATF Perfusion Bioreactors) Perfusion-SUB family using Alternating-Tangential-Flow (ATF) technology with SUBmerged HFF and inverted Thalia Alternating-Single-Use-Pump for cell retention. Includes CellRetention-300 BioBLU, CellRetention-500 CellVessel, CellRetention-2 SUBmerged, CellRetention-3 SUBmerged, CellRetention-13 SUBmerged, and CellRetention-30 SUBmerged models.
• CellRetention ATF-Traditional Series 32 Traditional ATF configuration P-SUBs with external pump configuration for mammalian cell perfusion, including CellRetention mini Traditional and CellRetention 3-13 Traditional models ranging from mini to 13 liters.
• CellRetention ATF-SUBmerged DIY Series 33 (ATF Kits) DIY kit series for SUBmerged ATF configuration allowing custom integration of components. Includes CellRetention-250 ATF kit Modular, CellRetention-PG13 ATF kit, and CellRetention-3 ATF kit for integration with Modular Process-Control-System, PG13 threaded vessels, and custom 3 liter SUBs.
• CellRetention ATF-External kit Series 34 External kit for ATF configuration allowing flexible system setup with externally-mounted pump arrangements for mammalian cell perfusion.
• Thalia Single-Use-Pump for ATF LASER-guided Alternating-Single-Use-Pump (A-SUP) for ATF perfusion systems, available in TM80-TM200 range, designed to master broth velocity from low harvest rates to high HFF cleaning velocities. Programmable to mimic mammalian heart function.
• Clio Single-Use-Pump for TFF LASER-guided OneWay-Single-Use-Pump (O-SUP) for TFF perfusion systems, available in CM80-CM200 range, copying mammalian heart function for accurate fluid control. Can be used customized as standalone for TFF applications.
• Clotho Drive Unit Dual-channel Drive Unit with 5 inch touch sensitive display for controlling Thalia and Clio SUPs, featuring built-in pressure sensors, LASER position detection, and OPC-UA communication. Controls stroke volume, number of strokes, and fluid velocity with real-time data presentation.
• Alagonia Vacuum/Pressure Pumps (Alagonia-8 and Alagonia-50) Low-noise twin and scroll diaphragm pumps supplying pressurized air or vacuum for Clotho Drive Units. Alagonia-8 suitable for SUP sizes M60, M80, M100; Alagonia-50 high-capacity model with 50 nl/min vacuum for SUP sizes M60 to M200. Integrated into stackable Hephaestus cabinets.
• Sarpedon Reservoir Two separated 1 liter metal storage chambers combined with manifolds for pressure/vacuum wave and pulse absorption, integrated into Hephaestus U2 cabinet for use with Clotho Drive Units.
• SepraPor Hollow-Fiber-Filter (HFF) Cartridges Hollow-Fiber-Filter cartridges from Meissner with 0.2 micrometer pore size for cell retention and perfusion filtration, available in various sizes (C012, C112, C224). Integrated with PerfuseCell's CellMembra and CellRetention perfusion systems.
• Clotho Process Control Software
• Customized & Configurable Product Development Services Customization and product design/development services where PerfuseCell develops custom products per customer specifications. Such projects become property of the company.

Quantifiable outcome

• Supports cell densities of 75-100 million cells/ml for mammalian cell cultures
• +2 more outcomes

Named customers2 records

Segments3 records

Ideal customer profiles3 records

Technology focussed Yes

API detail

Has API

No

API docs

API detail

Core technology

AI maturity

App detail

Integration11 records

Feature4 records

Partnerships

Five partnerships are on record, tiered core and flagship.

• MeissnercoreTechnology or IntegrationMeissner supplies SepraPor Hollow-Fiber-Filters (HFF) with pore size 0.2 µm used in PerfuseCell's CellMembra and CellRetention perfusion systems. SepraPor HFFs are integrated directly with the diaphragm pumps and SUBs.
• DASbox (Sartorius)coreTechnology or IntegrationCellMembra-300-DASbox integrates PerfuseCell's Clio diaphragm pump and SepraPor HFF directly into the DASbox bioreactor system for parallel perfusion operation.
• Modular (Parallel Bioreactors)coreTechnology or Integration250ml P-SUB designed to fit the Modular Process Control System for parallel perfusion operations.
• Cronus-PCScoreTechnology or IntegrationSister company within Stobbe Group providing Process Control Systems (PCS) for Process Development and small-scale manufacturing. Supplies hardware for Clotho, Lachesis, Atropos, Alagonia, Sarpedon products.
• Stobbe GroupflagshipStrategic or Co-development PartnerParent company of PerfuseCell-Cronus A/S. Comprises 5 sister companies (CerCell, PerfuseCell, Cronus-PCS, PumpCell, ProfileCell) all located at same address in Herlev, Denmark. Provides shared resources, manufacturing capabilities, and technology development.

Scale indicators3 records

Recent moves6 records

Expansion highlights6 records

Broad incumbents

• Sartorius: Global bioprocessing leader whose DASbox and Biostat bioreactor platforms are integration partners for PerfuseCell. Overlaps directly in upstream single-use bioreactors and competes for perfusion process-development business, but operates across a vastly wider product portfolio.
• Corning Life Sciences: Manufactures single-use bioreactors (Corning CellSTACK, Corning bioreactors, ASBC) for cell culture. Competes in single-use upstream and supplies vessels (CellVessel) that integrate with PerfuseCell's pump systems.
• Eppendorf: Manufactures benchtop and parallel bioreactor systems (DASbox predecessor, BioFlo) for process development and small-scale manufacturing, overlapping directly with PerfuseCell's lab/process-development perfusion niche.
• Cytiva (Danaher): Major upstream bioprocessing incumbent offering Xcellerex single-use bioreactors, ATF/perfusion systems, and filtration. Directly competes with PerfuseCell in single-use perfusion cell culture but at much larger scale.
• Merck KGaA / MilliporeSigma (Bioprocessing): Operates a major bioprocessing business with single-use bioreactors, filtration, and process development tools. Both a competitor and a validated customer (Merck named as PerfuseCell enterprise customer for CellMembra-300-DASbox).
• Thermo Fisher Scientific (Bioproduction): Operates a large bioproduction division including single-use bioreactors, filtration, and process analytics. Competes broadly with PerfuseCell in upstream bioprocessing equipment but at portfolio scale.

Direct peers

• ABEC: Provides single-use bioreactor solutions for biopharmaceutical manufacturing at multiple scales, including custom single-use systems. Competes with PerfuseCell's P-SUB offerings in single-use upstream bioprocessing.
• Repligen Corporation: Specializes in single-use bioprocessing including ATF perfusion systems, hollow-fiber filters, and chromatography. Closest direct competitor on perfusion cell-retention technology, competing head-to-head for high cell-density mAb process development customers.

Others

• Meissner Filtration Products: Supplies SepraPor Hollow-Fiber-Filters used in PerfuseCell's integrated perfusion systems. Strategic supplier rather than direct competitor, but adjacent in upstream filtration technology relevant to cell retention.

Emerging players

• PBS Biotech: Specializes in single-use, vertically scalable bioreactors for cell and gene therapy applications. Adjacent competitor focusing on emerging cell therapy manufacturing where PerfuseCell's high cell-density perfusion has potential relevance.

Market position

Strengths5 records

Weaknesses5 records

Competitive moat4 records

Key risks5 records

Key highlights7 records

Customer concentration

Compliance6 records

Revenue estimate

Valuation estimate

Number of profiles

Profiles4 records

Funding overview

Funding rounds

Investors

Funding detail is available on the Subscription and Enterprise plan.Contact sales →

M&A

Investments

M&A and investment is available on the Subscription and Enterprise plan.Contact sales →

Frequently asked questions about PerfuseCell