Biolegio
Biolegio is a Netherlands-based, ISO 13485-certified custom oligonucleotide synthesis company founded in 1996, serving life science researchers, pharmaceutical OEMs, and clinical diagnostic developers worldwide with custom DNA oligos, qPCR probes, and NGS-grade adapters distributed via webshop and a global partner network.
- Company typePrivate
- Founded1996
- HeadquartersNijmegen, Netherlands
- Headcount11–50
- GTM typeB2B
- OfferingHardware or Manufacturing
What Biolegio does
Biolegio B.V. is a privately held, founder-owned custom oligonucleotide synthesis company headquartered in Nijmegen, the Netherlands, founded in 1996 by Ruud van der Steen. The company manufactures custom DNA oligos (2–200+ bases with over 300 available modifications), a full range of qPCR probes (standard, MGB, MAX, LNA, XS, double-quenched, FRET), and NGS-grade adapters and indexing primers under its LegiSeq trademark. Production is anchored by a proprietary B-Pure synthesis protocol delivering >99.5% coupling efficiency across 200-base reactions and a dedicated NGS production pipeline maintaining cross-contamination below 0.1% (as low as 0.01%), with the Nijmegen facility certified to ISO 9001:2015 and ISO 13485:2016 for medical-device-grade oligonucleotide manufacture.
The company serves three distinct customer segments: individual and academic life science researchers ordering standard oligos through its webshop (standard DNA oligos shipped within 24 hours); pharmaceutical and clinical diagnostic OEMs requiring bulk synthesis, GMP-grade oligos, and guaranteed yields; and CRISPR researchers, served through a European distribution partnership with Synthego for synthetic sgRNA and Advanced RNA products. Revenue is generated primarily through per-order transactions on the webshop, prepaid credit accounts for recurring customers, OEM/B2B contracts with managed accounts, and distribution of partner RNA products. The company distributes globally through 14+ authorized distributors across more than 20 countries — including a dedicated logistics hub in Wayne, PA for the Americas — and operates as a sister entity to NimaGen under a shared holding structure and NetSuite ERP environment, with a combined headcount of approximately 40 colleagues across both organizations.
Biolegio firmographics
Firmographics- Name
- Biolegio
- Legal name
- Biolegio B.V.
- Website
- https://biolegio.com
- Company type
- Private
- Founded year
- 1996
- Operating status
- Operating
- Headcount range
- 11–50 employees
- Short description
- Biolegio is a Netherlands-based, ISO 13485-certified custom oligonucleotide synthesis company founded in 1996, serving life science researchers, pharmaceutical OEMs, and clinical diagnostic developers worldwide with custom DNA oligos, qPCR probes, and NGS-grade adapters distributed via webshop and a global partner network.
- Ownership category
- akta.pro rank
Biolegio industry classification
Industry- Product category
- Custom Oligonucleotide Synthesis
- NAICS
- Research and Development in Biotechnology (except Nanobiotechnology) (541714), Scientific Research and Development Services (5417)
- SIC
- Medicinal Chemicals & Botanical Products (2833), Laboratory Analytical Instruments (3826)
- akta.pro primary industry
- RNA Manufacturing & CMC (oligo synthesis, IVT, purification, formulation, QC) (HLAAADAF)
- akta.pro secondary industries
- RNA Chemistry & Oligonucleotide Engineering (backbones, modifications, stereochemistry) (HLAAADAE), Plasmid DNA & mRNA Manufacturing Services (HLAAAKAE), Molecular Biology & Genomics Instruments (PCR/qPCR, Sequencing, Microarrays) (HLAGAIAF)
Keywords
Where Biolegio is headquartered
LocationHeadquarters
- HQ city
- Nijmegen
- HQ country
- Netherlands
- HQ region
- Europe
Offices2 records
Markets served
Biolegio business model
Business model- GTM type
- B2B
- Offering type
- Hardware or Manufacturing
- Cost components
- Personnel, Operations, Supply Chain, Technology or R&D, Infrastructure, Marketing or Sales
Revenue model
- Custom Oligonucleotide Synthesis: Core business generating revenue through synthesis and sale of custom DNA oligos, qPCR probes, and modified oligonucleotides. Revenue derived from per-order transaction model with pricing based on length, modification complexity, purification level, and quantity.
- OEM & Bulk Synthesis Services: Large-scale research applications and industry production requirements with guaranteed delivered yields. Custom yields, specifications, and formats for clinical and molecular diagnostic products.
- RNA/CRISPR Products Partnership: Revenue from Synthego RNA products distributed in Europe through Biolegio, including synthetic sgRNA and Advanced RNA products.
- Oligo Dispensing Service: Pre-mixed assays and custom buffer services with premium pricing for convenience and reduced handling errors.
- Prepaid Oligo Service: Prepaid credit system allowing easy order placement and budget management for recurring customers.
Pricing tiers
| Model | Billing | Price |
|---|---|---|
| Unit Pricing | Pay-as-you-go | Standard DNA Oligos |
| Other | Monthly | Laboratory Technician Salary Range |
| Other | Monthly | Back-end Developer Salary Range |
| Unit Pricing | Pay-as-you-go | Standard qPCR Probes |
Go-to-market motion3 records
Distribution channels3 records
Marketing channels5 records
Biolegio product offering
Product offeringCore offering
Biolegio B.V. manufactures custom DNA and RNA oligonucleotides on a made-to-order basis, ranging from 2 to 200+ bases with more than 300 available chemical modifications, and ships standard oligos within 24 hours and purified/modified oligos within 4 business days. The offering spans qPCR probe families (standard dual-labelled, XS, MGB Eclipse, MAX, LNA, FRET, double quenched), the LegiSeq™ NGS Solutions line of adapters and indexing primers for Illumina library preparation, AMBeR sequencing primers, ReadyMax™ premixed assays for BD-MAX, and Synthego-distributed synthetic sgRNA and Advanced RNA for CRISPR applications. Services include custom 96/384-well plate synthesis, oligo dispensing, prepaid credit ordering, and collaborative custom solution projects.
Product overview
Biolegio is a custom oligonucleotide synthesis company offering a comprehensive portfolio of DNA and RNA synthesis products and services for life science research and molecular diagnostics. The core offering centers on DNA Synthesis (custom oligos 2-200+ bases with 300+ modifications) and Probes for qPCR (multiple probe types including MGB, MAX, LNA, FRET). The LegiSeq™ NGS Solutions brand provides pre-configured and custom NGS grade adapters and indexing primers for Illumina® sequencing platforms. RNA synthesis products are delivered through a partnership with Synthego, offering synthetic sgRNA and Advanced RNA for CRISPR applications. Service offerings include Custom Plate Synthesis, Oligo Dispensing, Custom Solutions, and Prepaid Oligo Services. The company operates primarily through its webshop platform with global distribution.
Differentiator
Problem solved
Functional benefit
Brands
- LegiSeq™ NGS: NGS grade oligonucleotides for library prep applications including adapters, indexing primers, and supplementary products for Illumina sequencing.
- LegiSeq™ Tagmentation Indexes
- LegiSeq™ Full Length UDI-UMI Adapters
- LegiSeq™ Universal Short Adapter & Primers
- ReadyMax™
- AMBeR Primers
Products and services
- DNA Synthesis (Custom DNA Oligos) Custom DNA oligonucleotide synthesis from 2 to 200+ bases with more than 300 available chemical modifications, produced under ISO 13485 and ISO 9001 quality standards in plate, strip, or tube formats. Standard DNA oligos ship within 24 hours; purified and modified oligos ship within 4 business days. It is intended for life science researchers, molecular diagnostic assay developers, and academic laboratories running PCR, sequencing, NGS library prep, and CRISPR experiments.
- Probes for qPCR A complete range of qPCR probes covering Standard Dual Labelled probes, XS probes, MGB Eclipse probes, MAX probes, Double Quenched probes, LNA probes, and FRET probes for LightCycler systems, with a full spectrum of dyes and quenchers. Probes ship within 5-7 working days and are intended for molecular diagnostic assay developers and life science researchers performing qPCR and RT-qPCR experiments.
- LegiSeq™ NGS Solutions Pre-configured and custom NGS-grade adapters and indexing primers for Illumina sequencing library preparation, covering Full Length UDI-UMI Adapters, Universal Short Adapter & Primers, and Tagmentation UDI Indexes. Produced via a dedicated NGS production pipeline that maintains cross-contamination below 0.1% and as low as 0.01%. Offered to NGS library preparation customers in research and clinical sequencing.
- ReadyMax™ for BD-MAX™ Custom premixed oligo mixes delivered in snap-in tubes for direct loading into BD-MAX™ cartridges, with predesigned assays (b-CAP, MTB, NP-1, NP-2, AP-1, AP-2, EP-1, EP-2, MP-1, MP-2, BB, HSV/VZV, Carba-1, Carba-2) and custom assay options. Designed for clinical laboratories running infectious disease testing on the BD-MAX automated cartridge system.
- Advanced RNA 50 / 75 / 100 (via Synthego partnership) Synthetic Advanced RNA products offered through Biolegio's European distribution partnership with Synthego, covering Advanced RNA 50 (20-50 nt), Advanced RNA 75 (51-75 nt), and Advanced RNA 100 (76-103 nt), shipping in 5-10 business days. Targeted at CRISPR and genome engineering researchers who need high-quality synthetic RNA for gene editing applications.
- Synthetic sgRNA (Synthego via Biolegio) Synthego synthetic single guide RNAs for CRISPR genome engineering, achieving up to 99% indel frequencies, with full-length 100-mer synthetic sgRNA available with optional site-specific chemical modifications. Distributed to European customers through Biolegio for CRISPR researchers and genome engineering labs.
- Bulk Scale Synthesis / GMP Oligos (OEM & B2B) Large-scale custom DNA oligo and qPCR probe synthesis with guaranteed delivered yields, offered under OEM and B2B supply terms with custom yields, specifications, and formats. Produced under ISO 13485 for clinical diagnostic product development and industry production requirements.
- AMBeR Sequencing Primers Sequencing primers featuring Advanced Mobility for Better Resolution (AMBeR) chemistry that dramatically improves Sanger sequencing reads in the first 50 bases. Targeted at life science researchers and Sanger sequencing users needing higher-quality reads near the sequencing primer binding region.
- Custom Plate Synthesis Service High-throughput, low-cost oligonucleotide synthesis delivered in 96 or 384-well plate format with options for single or multiple oligos per well, and custom concentrations and buffers. Available to researchers and diagnostic laboratories that need plate-based oligo orders with consistent formatting.
- Oligo Dispensing Service Premixed primer and probe assays dispensed according to customer specifications, with automated dispensing options that reduce test-to-test variation and handling errors. Used by diagnostic laboratories and assay developers who need pre-formatted, ready-to-run master mixes.
- Prepaid Oligo Service Credit-based prepaid oligo ordering service that enables recurring customers to easily manage oligo budgets and simplify order placement with immediate order capability. Suited for research labs and diagnostic customers with predictable, recurring oligo needs.
- Custom Solutions Service Collaborative custom-project development service for special projects and new products, combining Biolegio's expertise in custom oligo synthesis with customer-specific requirements. Engaged by customers needing bespoke synthesis specifications outside the standard catalogue.
- Assay Design & Development Services (NYtor referral) Referral service to NYtor for molecular diagnostic assay development, including primer and probe design, prototype development, and stability studies for qPCR, isothermal amplification (LAMP), and digital PCR applications. NYtor operates under EN ISO 13485:2016 Certified Quality Management System.
Quantifiable outcome
- Standard DNA oligos shipped within 24 hours
- +4 more outcomes
Companies that use Biolegio
Customer profileNamed customers5 records
Segments3 records
Ideal customer profiles4 records
Biolegio technology and API
TechnologyTechnology focussed No
API detail
- Has API
- No
- API docs
- API detail
Core technology
AI maturity
App detail
Feature4 records
Biolegio partnerships and signals
Strategic signalPartnerships
Ten partnerships are on record, tiered minor, core and major.
- EditCo Bio, Inc.minorIn 2024, EditCo Bio acquired Synthego's engineered cell solutions and enhanced guide RNA business. Biolegio refers customers to EditCo for Gene Knockout Kit v2 orders as they no longer supply this product.
- SynthegocoreBiolegio partners with Synthego to offer European customers access to their outstanding range of CRISPR genome engineering products including synthetic sgRNA, Advanced RNA products (50/75/100), and Gene Knockout Kits. This partnership provides swift, cost-effective, and convenient access to world-leading RNA chemistry and synthesis innovations.
- NYtorminorBiolegio recommends NYtor for assay design and development services. NYtor designs smart molecular detection assays for qPCR, isothermal amplification (LAMP), and digital PCR applications, with all work performed under EN ISO 13485:2016 Certified Quality Management System.
- NimaGencoreNimaGen is Biolegio's sister company within the same holding structure. Both organizations share a single NetSuite ERP environment. NimaGen develops and supplies reagents and kits for molecular research enabling laboratories and hospitals to detect and analyze diseases.
- Amerigo ScientificmajorAuthorized distributor for United States. Provides local customer support, technical assistance, and order placement for Biolegio products in the US market.
- Dominique DutschermajorAuthorized distributor for France. Provides local customer support, technical assistance, and order placement for Biolegio products.
- TeknokromamajorAuthorized distributor for Spain. Provides local customer support, technical assistance, and order placement for Biolegio products.
- AntiselmajorAuthorized distributor for Greece and Romania. Provides local customer support, technical assistance, and order placement for Biolegio products.
- BiogenomedmajorAuthorized distributor for Thailand. Provides local customer support, technical assistance, and order placement for Biolegio products.
- Lablife NordicmajorAuthorized distributor for Denmark, Finland, and Sweden. Provides local customer support, technical assistance, and order placement for Biolegio products in Nordic markets.
Scale indicators6 records
Recent moves6 records
Expansion highlights5 records
Biolegio competitors and assessment
Company assessmentEmerging players
- Synthego: Synthego is a leading synthetic RNA and CRISPR reagent provider whose products Biolegio distributes in Europe. Overlap with Biolegio's CRISPR/sgRNA product line and partial overlap in custom oligonucleotide chemistry make it a relevant peer (and partner).
- Twist Bioscience: Twist Bioscience uses silicon-based DNA synthesis to produce oligos, genes, and NGS enrichment panels. While its platform technology differs, it competes with Biolegio's high-throughput NGS adapter, oligo pool, and custom synthesis offerings.
Broad incumbents
- GenScript: GenScript provides custom oligo synthesis, gene synthesis, and broader life science research services globally. Overlaps with Biolegio on standard DNA oligos and NGS-related services, and is a comparable global-distribution model.
- Eurofins Genomics: Eurofins Genomics operates a large-scale custom oligo and sequencing services business as part of the broader Eurofins Scientific testing portfolio. It competes with Biolegio on standard oligos, qPCR probes, and OEM supply but at materially greater scale.
- Thermo Fisher Scientific (Oligo Services): Thermo Fisher offers custom oligonucleotide manufacturing through its Life Sciences Solutions segment (including the former Dharmacon business), competing broadly across research oligos, probes, and OEM supply with significantly greater resources.
- Agilent Technologies (Oligo Manufacturing): Agilent offers custom oligonucleotide manufacturing and SureSelect NGS reagent kits. It overlaps with Biolegio's NGS-grade oligos and oligo services as part of a much broader analytical instruments and life sciences portfolio.
- TriLink BioTechnologies (Maravai): TriLink manufactures oligonucleotides, mRNA, and nucleic acid reagents for therapeutic and diagnostic customers, including GMP-grade oligos. Comparable to Biolegio's bulk synthesis and OEM/GMP offering, but with deeper therapeutic oligo capability.
Direct peers
- Integrated DNA Technologies (IDT): IDT is the leading global custom oligonucleotide synthesis provider, offering DNA/RNA oligos, qPCR probes, NGS adapters, and gene synthesis. It is Biolegio's most direct competitor across Biolegio's entire product portfolio, including standard oligos, NGS, and OEM.
- LGC Biosearch Technologies: Biosearch Technologies is a specialist oligo and probe manufacturer known for custom DNA probes, fluorophore chemistry, and qPCR/NGS reagents. Directly comparable to Biolegio's qPCR probe and NGS adapter offerings.
Others
- Biolytic Lab Performance: Biolytic designs and sells oligonucleotide synthesizers and synthesis reagents. It is an enabling/ecosystem peer: Biolegio's synthesis capability is built on phosphoramidite chemistry instruments like those Biolytic produces.
Market position
Strengths5 records
Weaknesses4 records
Competitive moat4 records
Key risks6 records
Key highlights7 records
Customer concentration
Biolegio social profiles
Digital presenceBiolegio compliance and trust
Trust signalCompliance2 records
Biolegio financial estimates
Financial estimateRevenue estimate
Valuation estimate
Biolegio leadership team
Management profileNumber of profiles
Profiles4 records
Biolegio funding detail
Funding detailFunding overview
Funding rounds
Investors
Funding detail is available on the Subscription and Enterprise plan.Contact sales →
Biolegio M&A and investment
M&A and investmentM&A
Investments
M&A and investment is available on the Subscription and Enterprise plan.Contact sales →
Frequently asked questions about Biolegio
What does Biolegio do?
Biolegio B.V. manufactures custom DNA and RNA oligonucleotides on a made-to-order basis, ranging from 2 to 200+ bases with more than 300 available chemical modifications, and ships standard oligos within 24 hours and purified/modified oligos within 4 business days. The offering spans qPCR probe families (standard dual-labelled, XS, MGB Eclipse, MAX, LNA, FRET, double quenched), the LegiSeq™ NGS Solutions line of adapters and indexing primers for Illumina library preparation, AMBeR sequencing primers, ReadyMax™ premixed assays for BD-MAX, and Synthego-distributed synthetic sgRNA and Advanced RNA for CRISPR applications. Services include custom 96/384-well plate synthesis, oligo dispensing, prepaid credit ordering, and collaborative custom solution projects.
Is Biolegio a public or private company?
Biolegio is a private company. It is classified as founder individual operated bootstrapped and is currently operating.
When was Biolegio founded?
Biolegio was founded in 1996. It employs 11 to 50 people.
Where is Biolegio based?
Biolegio is headquartered in Nijmegen, Netherlands, in the Europe region.
How does Biolegio make money?
Five revenue lines are on record. Custom Oligonucleotide Synthesis are the primary driver. The others are OEM & Bulk Synthesis Services, RNA/CRISPR Products Partnership, oligo Dispensing Service and prepaid Oligo Service.
Who are Biolegio's main competitors?
Emerging players on record are Synthego and Twist Bioscience. Broad incumbents are GenScript, Eurofins Genomics, Thermo Fisher Scientific (Oligo Services), Agilent Technologies (Oligo Manufacturing) and TriLink BioTechnologies (Maravai). Direct peers are Integrated DNA Technologies (IDT) and LGC Biosearch Technologies. Biolytic Lab Performance is listed as an others.
Does Biolegio have an API?
No public API is recorded for Biolegio.
What industry is Biolegio in?
Biolegio's product category is Custom Oligonucleotide Synthesis. Its primary akta.pro industry code is HLAAADAF, RNA Manufacturing & CMC (oligo synthesis, IVT, purification, formulation, QC), with a secondary code of HLAAADAE, RNA Chemistry & Oligonucleotide Engineering (backbones, modifications, stereochemistry). Its NAICS code is 541714 and its SIC code is 2833.